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![Western Blot: PHF6 Antibody [NB100-68262] - Detection of Human PHF6 on HeLa whole cell lysate.PHF6 was also immunoprecipitated by rabbit anti-PHF6 antibodies NB100-68260 and NB100-68261.](http://images.novusbio.com/fullsize/PHF6-Antibody-Western-Blot-NB100-68262-img0004.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PHF6 Antibody [NB100-68262] - Detection of Human PHF6 on HeLa whole cell lysate.PHF6 was also immunoprecipitated by rabbit anti-PHF6 antibodies NB100-68260 and NB100-68261.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: PHF6 Antibody [NB100-68262] - Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti- PHF6 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunohistochemistry-NB100-68262-img0006.jpg)
![Western Blot: PHF6 Antibody [NB100-68262] - Image from a customer review testing on 293T cells.](http://images.novusbio.com/fullsize/PHF6-Antibody-Western-Blot-NB100-68262-img0003.jpg)
![Immunohistochemistry-Paraffin: PHF6 Antibody [NBP1-88646] - Staining in human epididymis and liver tissues using anti-PHF6 antibody. Corresponding PHF6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunohistochemistry-Paraffin-NBP1-88646-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PHF6 Antibody [NBP1-88646] - Staining in human epididymis and liver tissues using anti-PHF6 antibody. Corresponding PHF6 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: PHF6 Antibody [NBP1-88646] - Analysis in human cell line EFO-21.](http://images.novusbio.com/fullsize/PHF6-Antibody-Western-Blot-NBP1-88646-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: PHF6 Antibody [NBP1-88646] - Staining of human cell line U-251MG shows positivity in nucleus and nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88646-img0004.jpg)
![Immunohistochemistry-Paraffin: PHF6 Antibody [NBP1-88646] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunohistochemistry-Paraffin-NBP1-88646-img0017.jpg)
![Immunohistochemistry-Paraffin: PHF6 Antibody [NBP1-88646] - Staining of human epididymis shows high expression.](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunohistochemistry-Paraffin-NBP1-88646-img0010.jpg)
![Immunohistochemistry-Paraffin: PHF6 Antibody [NBP1-88646] - Staining of human tonsil shows strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunohistochemistry-Paraffin-NBP1-88646-img0014.jpg)
![Immunohistochemistry-Paraffin: PHF6 Antibody [NBP1-88646] - Staining of human cerebral cortex shows moderate nuclear and nucleolar positivity in neurons.](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunohistochemistry-Paraffin-NBP1-88646-img0015.jpg)
![Immunohistochemistry-Paraffin: PHF6 Antibody [NBP1-88646] - Staining of human testis shows strong nuclear positivity in Leydig cells.](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunohistochemistry-Paraffin-NBP1-88646-img0016.jpg)
![Western Blot: PHF6 Antibody [NB100-68260] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PHF6 antibody NB100-68260 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PHF6 was also immunoprecipitated by rabbit anti-PHF6 antibodies NB100-68261 and NB100-68262, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/PHF6-Antibody-Western-Blot-NB100-68260-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PHF6 Antibody [NB100-68260] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PHF6 antibody NB100-68260 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PHF6 was also immunoprecipitated by rabbit anti-PHF6 antibodies NB100-68261 and NB100-68262, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: PHF6 Antibody [NB100-68260] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PHF6 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunohistochemistry-Paraffin-NB100-68260-img0001.jpg)
![Western Blot: PHF6 Antibody [NB100-68261] - Detection of Human PHF6 on HeLa whole cell lysate using NB100-68261. PHF6 was also immunoprecipitated by rabbit anti-PHF6 antibodies NB100-68260 and NB100-68262.](http://images.novusbio.com/fullsize/PHF6-Antibody-Western-Blot-NB100-68261-img0004.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PHF6 Antibody [NB100-68261] - Detection of Human PHF6 on HeLa whole cell lysate using NB100-68261. PHF6 was also immunoprecipitated by rabbit anti-PHF6 antibodies NB100-68260 and NB100-68262.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: PHF6 Antibody [NB100-68261] - Section of human breast carcinoma. Affinity purified rabbit anti- PHF6 used at 1:200 (1 ug/mL). Detection by DAB.](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunohistochemistry-NB100-68261-img0005.jpg)
![Western Blot: PHF6 Antibody [NBP2-19799] - Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PHF6-Antibody-Western-Blot-NBP2-19799-img0001.jpg "Western Blot: PHF6 Antibody [NBP2-19799] - Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: PHF6 Antibody [NBP2-19799] - Analysis of paraformaldehyde-fixed MCF-7, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19799-img0002.jpg)
![Western Blot: PHF6 Antibody (8F24) [H00084295-M01-100ug] - Detection against Immunogen (36.63 KDa) .](http://images.novusbio.com/fullsize/PHF6-Antibody-8F24-Western-Blot-H00084295-M01-100ug-img0002.jpg "Western Blot: PHF6 Antibody (8F24) [H00084295-M01-100ug] - Detection against Immunogen (36.63 KDa) .")
![ELISA: PHF6 Antibody (8F24) [H00084295-M01-100ug] - Detection limit for recombinant GST tagged PHF6 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PHF6-Antibody-8F24-ELISA-H00084295-M01-100ug-img0001.jpg)
![Western Blot: PHF6 Overexpression Lysate (Adult Normal) [NBP2-08751] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PHF6.](http://images.novusbio.com/fullsize/PHF6-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08751-img0001.jpg "Western Blot: PHF6 Overexpression Lysate (Adult Normal) [NBP2-08751] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PHF6.")
![Western Blot: PHF6 Overexpression Lysate (Adult Normal) [NBP2-06017] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PHF6.](http://images.novusbio.com/fullsize/PHF6-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06017-img0001.jpg "Western Blot: PHF6 Overexpression Lysate (Adult Normal) [NBP2-06017] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PHF6.")
![Western Blot: PHF6 Antibody [NBP2-95270] - Western blot analysis was performed by loading 25 ug of various whole cell lysates probed with Rabbit anti PHF6 (NBP2-95270) at 1:1000 dilution. Arrow points to PHF6 (molecular weight 41 kDa).](http://images.novusbio.com/fullsize/PHF6-Antibody-Western-Blot-NBP2-95270-img0001.jpg "Western Blot: PHF6 Antibody [NBP2-95270] - Western blot analysis was performed by loading 25 ug of various whole cell lysates probed with Rabbit anti PHF6 (NBP2-95270) at 1:1000 dilution. Arrow points to PHF6 (molecular weight 41 kDa).")
![Immunohistochemistry-Paraffin: PHF6 Antibody [NBP2-97699] - Immunochemical staining of human PHF6 in human epididymis with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunohistochemistry-Paraffin-NBP2-97699-img0001.jpg "Immunohistochemistry-Paraffin: PHF6 Antibody [NBP2-97699] - Immunochemical staining of human PHF6 in human epididymis with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.")