| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52669-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20816 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-23979 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-24334 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-24334 | |||
![Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM-1/CD56 on human brain tissue lysates with Rabbit anti-NCAM/CD56 antibody at 1/2,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 95 kDa Observed band size: 140/180 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Western-Blot-NBP2-66968-img0009.jpg "Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM-1/CD56 on human brain tissue lysates with Rabbit anti-NCAM/CD56 antibody at 1/2,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 95 kDa Observed band size: 140/180 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Staining NCAM in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunocytochemistry-Immunofluorescence-NBP2-66968-img0007.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-NCAM-1/CD56 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0011.jpg)
![Flow Cytometry: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of SH-SY-5Y cells with NCAM antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Flow-Cytometry-NBP2-66968-img0001.jpg)
![Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM on different lysates using anti-NCAM antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: Human brain](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Western-Blot-NBP2-66968-img0006.jpg)
![Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM-1/CD56 on SH-SY5Y cell lysates with Rabbit anti-NCAM-1/CD56 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 95 kDa Observed band size: 120/140 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Western-Blot-NBP2-66968-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Staining NCAM in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunocytochemistry-Immunofluorescence-NBP2-66968-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Staining NCAM in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunocytochemistry-Immunofluorescence-NBP2-66968-img0003.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded human tonsil tissue using anti-NCAM antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0004.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded zebrafish kidney tissue using anti-NCAM antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0005.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded human tonsil tissue using anti-NCAM-1/CD56 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0010.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP2-38452] - Staining in human cerebral cortex and skin tissues.. Corresponding NCAM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-Immunohistochemistry-Paraffin-NBP2-38452-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP2-38452] - Staining in human cerebral cortex and skin tissues.. Corresponding NCAM1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: NCAM-1/CD56 Antibody [NBP2-38452] - Analysis in human brain tissue.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-Western-Blot-NBP2-38452-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: NCAM-1/CD56 Antibody [NBP2-38452] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38452-img0004.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP2-38452] - Staining of human skin shows no positivity in keratinocytes.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-Immunohistochemistry-Paraffin-NBP2-38452-img0015.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP2-38452] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-Immunohistochemistry-Paraffin-NBP2-38452-img0012.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP2-38452] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-Immunohistochemistry-Paraffin-NBP2-38452-img0013.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP2-38452] - Staining of human heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-Immunohistochemistry-Paraffin-NBP2-38452-img0014.jpg)
![Western Blot: NCAM-1/CD56 Antibody (735) - Chimeric [NBP2-52669] - Rat brain lysate was resolved on a 10% SDS PAGE gel and blots probed with NBP2-52669 at 2 ug/ml before being detected by a secondary antibody.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-735-Chimeric-Western-Blot-NBP2-52669-img0003.jpg "Western Blot: NCAM-1/CD56 Antibody (735) - Chimeric [NBP2-52669] - Rat brain lysate was resolved on a 10% SDS PAGE gel and blots probed with NBP2-52669 at 2 ug/ml before being detected by a secondary antibody.")
![Immunohistochemistry: NCAM-1/CD56 Antibody (735) - Chimeric [NBP2-52669] - Formalin fixed rat spinal chord slices were were stained with a NBP2-52669 at 3 ug/ml.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-735-Chimeric-Immunohistochemistry-NBP2-52669-img0004.jpg)
![Immunohistochemistry: NCAM-1/CD56 Antibody (735) - Chimeric [NBP2-52669] - Formalin fixed guinea pig brain slices were were stained with a NBP2-52669 at 3 ug/ml.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-735-Chimeric-Immunohistochemistry-NBP2-52669-img0001.jpg)
