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![Western Blot: Lgr5/GPR49 Antibody (4H8) [NBP3-15237] - Positive WB detected in: SH-SY5Y whole cell lysate, Rat brain tissue. All lanes: Lgr5/GPR49 antibody at 1:1500. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 100, 98, 93 KDa. Observed band size: 100 KDa.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-4H8-Western-Blot-NBP3-15237-img0002.jpg "Western Blot: Lgr5/GPR49 Antibody (4H8) [NBP3-15237] - Positive WB detected in: SH-SY5Y whole cell lysate, Rat brain tissue. All lanes: Lgr5/GPR49 antibody at 1:1500. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 100, 98, 93 KDa. Observed band size: 100 KDa.")
![Flow Cytometry: Lgr5/GPR49 Antibody (4H8) [NBP3-15237] - Overlay histogram showing HepG2 cells stained with Lgr5/GPR49 antibody (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-4H8-Flow-Cytometry-NBP3-15237-img0001.jpg)
![Western Blot: Lgr5/GPR49 Antibody (9J9B8) [NBP3-15445] - Western blot analysis of extracts of various cell lines, using Lgr5/GPR49 Rabbit mAb (NBP3-15445) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-9J9B8-Western-Blot-NBP3-15445-img0001.jpg "Western Blot: Lgr5/GPR49 Antibody (9J9B8) [NBP3-15445] - Western blot analysis of extracts of various cell lines, using Lgr5/GPR49 Rabbit mAb (NBP3-15445) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: GPR49/LGR5 Overexpression Lysate (Adult Normal) [NBL1-12509] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GPR49/LGR5.](http://images.novusbio.com/fullsize/GPR49-LGR5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12509-img0002.jpg "Western Blot: GPR49/LGR5 Overexpression Lysate (Adult Normal) [NBL1-12509] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GPR49/LGR5.")