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![N/A Granzyme B [Biotin]](http://images.novusbio.com/fullsize2/DATA_Granzyme_B_DY1865_ELISA_1729.jpg "N/A Granzyme B [Biotin]")
![N/A Granzyme B [Biotin]](http://images.novusbio.com/fullsize2/DATA_Granzyme_B_DY290605_ELISA_2415.jpg "N/A Granzyme B [Biotin]")
![Flow Cytometry: Granzyme B Antibody (B18.1) [NBP1-97525] - An intracellular stain was performed on U937 cells with Granzyme B [B18.1] Antibody NBP1-97525AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-B18-1-Flow-Cytometry-NBP1-97525-img0006.jpg "Flow Cytometry: Granzyme B Antibody (B18.1) [NBP1-97525] - An intracellular stain was performed on U937 cells with Granzyme B [B18.1] Antibody NBP1-97525AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Western Blot: Granzyme B Antibody (B18.1) [NBP1-97525] - Detection of recombinant granzyme B. Granzyme B migrates as a 27 kDa species. Method: Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B antibody B18.1 at 1ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-B18-1-Western-Blot-NBP1-97525-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Granzyme B Antibody (B18.1) [NBP1-97525] - Human T cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hr at room temperature (RT) with 20 ug/ml of B18.4 antibody in PBS, 0.1% BSA. After rinsing in PBS, a FITC-conjugated anti-mouse IgG was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-B18-1-Immunocytochemistry-Immunofluorescence-NBP1-97525-img0005.jpg)
![Flow Cytometry: Granzyme B Antibody (B18.1) [NBP1-97525] - [FITC] Detection of granzyme B in permeabilized human CD8+ T lymphocytes (filled profile). Isotype control in combination with a secondary FITC-labeled antibody is shown as control. Method: Permeabilized human T cells (5 x 10^5).](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-B18-1-Flow-Cytometry-NBP1-97525-img0002.jpg)