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![Western Blot: Cofilin [p Ser3] Antibody [NBP3-05394] - Western blot analysis of extracts of NIH/3T3 cells, using Cofilin pAb (NBP3-05394) at 1:1000 dilution or CFL1 antibody (A1704).NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Cofilin-[p-Ser3]-Antibody-Western-Blot-NBP3-05394-img0003.jpg "Western Blot: Cofilin [p Ser3] Antibody [NBP3-05394] - Western blot analysis of extracts of NIH/3T3 cells, using Cofilin pAb (NBP3-05394) at 1:1000 dilution or CFL1 antibody (A1704).NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: Cofilin [p Ser3] Antibody [NBP3-05394] - Immunofluorescence analysis of methanol-fixed HeLa cells using Cofilin antibody (NBP3-05394).](http://images.novusbio.com/fullsize/Cofilin-[p-Ser3]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05394-img0002.jpg)
![Immunohistochemistry-Paraffin: Cofilin [p Ser3] Antibody [NBP3-05394] - Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Cofilin antibody (NBP3-05394).](http://images.novusbio.com/fullsize/Cofilin-[p-Ser3]-Antibody-Immunohistochemistry-Paraffin-NBP3-05394-img0001.jpg)
![Western Blot: Cofilin Antibody (1C1) [NBP1-21044] - The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cofilin 1(1:1000) antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: K562 cell lysateLane 3.: MCF7 cell lysateLane 4.: LnCap cell lysateLane 5.: PC3 cell lysate](http://images.novusbio.com/fullsize/Cofilin-Antibody-1C1-Western-Blot-NBP1-21044-img0007.jpg "Western Blot: Cofilin Antibody (1C1) [NBP1-21044] - The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cofilin 1(1:1000) antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: K562 cell lysateLane 3.: MCF7 cell lysateLane 4.: LnCap cell lysateLane 5.: PC3 cell lysate")
![Immunocytochemistry/Immunofluorescence: Cofilin Antibody (1C1) [NBP1-21044] - Analysis of Cofilin1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Cofilin1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Cofilin-Antibody-1C1-Immunocytochemistry-Immunofluorescence-NBP1-21044-img0006.jpg)
![Western Blot: Cofilin Antibody [NBP3-23230] - Western blot of Cofilin in jurkat cells. The blots were untreated (lanes 1 & 4) or treated (lanes 2, 3, 5 & 6) with lambda phosphatase. In lanes 3 & 6, the phosphatase was inhibited with phospho-Cofilin (Ser-3) peptide. The blots were probed with anti-Cofilin (Ser-3) phospho-specific (lanes 1-3) or anti-Cofilin (N-terminus) (lanes 4-6).](http://images.novusbio.com/fullsize/nbp3-23230_rabbit-cofilin-pab-19920231759441.jpg "Western Blot: Cofilin Antibody [NBP3-23230] - Western blot of Cofilin in jurkat cells. The blots were untreated (lanes 1 & 4) or treated (lanes 2, 3, 5 & 6) with lambda phosphatase. In lanes 3 & 6, the phosphatase was inhibited with phospho-Cofilin (Ser-3) peptide. The blots were probed with anti-Cofilin (Ser-3) phospho-specific (lanes 1-3) or anti-Cofilin (N-terminus) (lanes 4-6).")
![Western Blot: Cofilin [p Ser3] Antibody [NBP3-23231] - Western blot of Cofilin in jurkat cells. The blots were untreated (lanes 1 & 4) or treated (lanes 2, 3, 5 & 6) with lambda phosphatase. In lanes 3 & 6, the phosphatase was inhibited with phospho-Cofilin (Ser-3) peptide. The blots were probed with anti-Cofilin (Ser-3) phospho-specific (lanes 1-3) or anti-Cofilin (N-terminus) (lanes 4-6).](http://images.novusbio.com/fullsize/nbp3-23231_rabbit-cofilin-p-ser3-pab-199202312403712.jpg "Western Blot: Cofilin [p Ser3] Antibody [NBP3-23231] - Western blot of Cofilin in jurkat cells. The blots were untreated (lanes 1 & 4) or treated (lanes 2, 3, 5 & 6) with lambda phosphatase. In lanes 3 & 6, the phosphatase was inhibited with phospho-Cofilin (Ser-3) peptide. The blots were probed with anti-Cofilin (Ser-3) phospho-specific (lanes 1-3) or anti-Cofilin (N-terminus) (lanes 4-6).")
![Western Blot: Cofilin [p Ser3] Antibody [NBP3-13302] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin (phospho Ser3) antibody (NBP3-13302) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cofilin-[p-Ser3]-Antibody-Western-Blot-NBP3-13302-img0002.jpg "Western Blot: Cofilin [p Ser3] Antibody [NBP3-13302] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin (phospho Ser3) antibody (NBP3-13302) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: Cofilin [p Ser3] Antibody [NBP3-13302] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin (phospho Ser3) antibody (NBP3-13302) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cofilin-[p-Ser3]-Antibody-Western-Blot-NBP3-13302-img0001.jpg)
![ELISA: Mouse Cofilin ELISA Kit (Colorimetric) [NBP2-75252] - Samples were spiked with high concentrations of Mouse Cofilin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75252_mouse-cofilin-elisa-kit-colorimetric-132202416254020.jpg "ELISA: Mouse Cofilin ELISA Kit (Colorimetric) [NBP2-75252] - Samples were spiked with high concentrations of Mouse Cofilin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Cofilin ELISA Kit (Colorimetric) [NBP2-75252] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75252_mouse-cofilin-elisa-kit-colorimetric-4320249115649.jpg)
![ELISA: Rat Cofilin ELISA Kit (Colorimetric) [NBP2-75253] - Samples were spiked with high concentrations of Rat Cofilin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75253_rat-cofilin-elisa-kit-colorimetric-132202416121378.jpg "ELISA: Rat Cofilin ELISA Kit (Colorimetric) [NBP2-75253] - Samples were spiked with high concentrations of Rat Cofilin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Cofilin ELISA Kit (Colorimetric) [NBP2-75253] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75253_rat-cofilin-elisa-kit-colorimetric-432024856778.jpg)
![ELISA: Human Cofilin ELISA Kit (Colorimetric) [NBP2-75251] - Samples were spiked with high concentrations of Human Cofilin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75251_human-cofilin-elisa-kit-colorimetric-13220241626182.jpg "ELISA: Human Cofilin ELISA Kit (Colorimetric) [NBP2-75251] - Samples were spiked with high concentrations of Human Cofilin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Cofilin ELISA Kit (Colorimetric) [NBP2-75251] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75251_human-cofilin-elisa-kit-colorimetric-432024973610.jpg)