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![Western Blot: CREB [p Ser133] Antibody [NB300-273] - Rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~45 kDa CREB phosphorylated at Ser133 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase lambda-Ptase, 800 units/1mg protein for 30 min).](http://images.novusbio.com/fullsize/CREB-[p-Ser133]-Antibody-Western-Blot-NB300-273-img0007.jpg "Western Blot: CREB [p Ser133] Antibody [NB300-273] - Rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~45 kDa CREB phosphorylated at Ser133 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase lambda-Ptase, 800 units/1mg protein for 30 min).")
![Immunohistochemistry-Frozen: CREB [p Ser133] Antibody [NB300-273] - IHC Analysis of pCREB S133 (red) in frozen sections from mouse piriform cortex. Cell nuclei are visualized with DAPI DNA stain (blue). NB300-273 was used at a dilution of 1:1000 and incubated for 48 hours at 4C. No antigen retrieval step was performed.](http://images.novusbio.com/fullsize/CREB-[p-Ser133]-Antibody-Immunohistochemistry-Frozen-NB300-273-img0006.jpg)
![Immunohistochemistry: CREB [p Ser 133] Antibody (SR1800) [NBP3-21507] - Immunohistochemical analysis of paraffin-embedded human gastric carcinoma, using NBP3-21507.](http://images.novusbio.com/fullsize/nbp3-21507_rabbit-creb-p-ser-133-mab-sr1800-57202316494290.jpg "Immunohistochemistry: CREB [p Ser 133] Antibody (SR1800) [NBP3-21507] - Immunohistochemical analysis of paraffin-embedded human gastric carcinoma, using NBP3-21507.")
![Western Blot: CREB [p Ser 133] Antibody (SR1800) [NBP3-21507] - Western blot analysis using NBP3-21507 in HeLa cell lysates treated with Calyculin A.](http://images.novusbio.com/fullsize/nbp3-21507_rabbit-creb-p-ser-133-mab-sr1800-6720239533770.jpg)
![Western Blot: CREB Antibody [NB100-87001] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. NB100-87001 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.](http://images.novusbio.com/fullsize/CREB-Antibody-Western-Blot-NB100-87001-img0004.jpg "Western Blot: CREB Antibody [NB100-87001] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. NB100-87001 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.")
![Immunohistochemistry-Paraffin: CREB Antibody [NB100-87001] - Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-CREB used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CREB-Antibody-Immunohistochemistry-NB100-87001-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CREB [p Ser133] Antibody (M415) [NBP3-23244] - Immunocytochemical labeling of phosphorylated CREB in calyculin A-treated A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40 before labeling with mouse monoclonal CREB (Ser-133). The antibody was detected using goat anti-mouse DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23244_mouse-creb-p-ser133-mab-m415-19920231915415.jpg "Immunocytochemistry/Immunofluorescence: CREB [p Ser133] Antibody (M415) [NBP3-23244] - Immunocytochemical labeling of phosphorylated CREB in calyculin A-treated A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40 before labeling with mouse monoclonal CREB (Ser-133). The antibody was detected using goat anti-mouse DyLight(R) 594.")
![Western Blot: CREB [p Ser133] Antibody (M415) [NBP3-23244] - Western blot analysis of human A431 cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3 & 5) then the blots were treated with lambda phosphatase (lanes 2, 4 & 6). The blots were probed with anti-CREB (N-terminal region) (lanes 1 & 2), anti-CREB (Ser-133) (lanes 3 & 4), and anti-CREB (Ser-271) (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23244_mouse-creb-p-ser133-mab-m415-199202312523628.jpg)
![Immunocytochemistry/Immunofluorescence: CREB [p Ser271] Antibody [NBP3-23250] - Immunocytochemical labeling of phosphorylated CREB in control and calyculin A-treated A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40 before labeling with rabbit polyclonal CREB (Ser-271). The antibody was detected using goat anti-rabbit DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23250_rabbit-creb-p-ser271-pab-1992023199286.jpg "Immunocytochemistry/Immunofluorescence: CREB [p Ser271] Antibody [NBP3-23250] - Immunocytochemical labeling of phosphorylated CREB in control and calyculin A-treated A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40 before labeling with rabbit polyclonal CREB (Ser-271). The antibody was detected using goat anti-rabbit DyLight(R) 594.")
![Western Blot: CREB [p Ser271] Antibody [NBP3-23250] - Western blot analysis of human A431 cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-CREB (N-terminal region) (lanes 1 & 2), anti-CREB (Ser-133) (lanes 3 & 4), and anti-CREB (Ser-271) (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23250_rabbit-creb-p-ser271-pab-189202317355213.jpg)
![Western Blot: CREB Antibody [NBP3-23243] - Western blot analysis of human A431 cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3 & 5) then the blots were treated with lambda phosphatase (lanes 2, 4 & 6). The blots were probed with anti-CREB (N-terminal region) (lanes 1 & 2), anti-CREB (Ser-133) (lanes 3 & 4), and anti-CREB (Ser-271) (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23243_rabbit-creb-pab-19920231856929.jpg "Western Blot: CREB Antibody [NBP3-23243] - Western blot analysis of human A431 cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3 & 5) then the blots were treated with lambda phosphatase (lanes 2, 4 & 6). The blots were probed with anti-CREB (N-terminal region) (lanes 1 & 2), anti-CREB (Ser-133) (lanes 3 & 4), and anti-CREB (Ser-271) (lanes 5 & 6).")
![Immunocytochemistry/Immunofluorescence: CREB Antibody [NBP3-23243] - Immunocytochemical labeling of CREB in A431 that were fixed in paraformaldehyde and permeabilized using NP-40. The cells were labeled with rabbit polyclonal CREB (N-terminal region). The antibody was detected using goat anti-rabbit DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23243_rabbit-creb-pab-199202312523630.jpg)
![Western Blot: CREB [p Ser129] Antibody [NBP3-05463] - Western blot analysis of extracts of 293T cells, using CREB antibody (NBP3-05463) at 1:500 dilution.293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/CREB-[p-Ser129]-Antibody-Western-Blot-NBP3-05463-img0001.jpg "Western Blot: CREB [p Ser129] Antibody [NBP3-05463] - Western blot analysis of extracts of 293T cells, using CREB antibody (NBP3-05463) at 1:500 dilution.293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: CREB [p Ser129] Antibody [NBP3-05463] - Western blot analysis of extracts of C6 cells, using CREB antibody (NBP3-05463) at 1:500 dilution.C6 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CREB [p Ser129] Antibody-Western Blot-NBP3-05463-img0002.jpg)
![Immunohistochemistry-Paraffin: CREB [p Ser142] Antibody [NBP3-05412] - Immunohistochemistry of paraffin-embedded human breast carcinoma using CREB antibody (NBP3-05412). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CREB [p Ser142] Antibody-Immunohistochemistry-Paraffin-NBP3-05412-img0001.jpg "Immunohistochemistry-Paraffin: CREB [p Ser142] Antibody [NBP3-05412] - Immunohistochemistry of paraffin-embedded human breast carcinoma using CREB antibody (NBP3-05412). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.")