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![Functional: Recombinant Human, Mouse Flagellin TLR5 Mutant His Protein [NBP3-18159] - Flagellin (rec.) and Flagellin (NLRC4 Mutant) (rec.) activate TLR5 in vivo.Method: Mice WT are injected i.v. with 1ug of Flagellin (rec.) (=WT) or Flagellin (NLRC4 Mutant) (rec.). After 2 hours, levels of CCL20 in the serum are measured by ELISA. As control, WT mice are injected i.v. with 1ug of Flagellin (TLR5 Mutant) (rec.). PBS injected mice are used as negative control.](http://images.novusbio.com/fullsize/Recombinant-Human--Mouse-Flagellin-TLR5-Mutant-His-Protein-Functional-NBP3-18159-img0001.jpg "Functional: Recombinant Human, Mouse Flagellin TLR5 Mutant His Protein [NBP3-18159] - Flagellin (rec.) and Flagellin (NLRC4 Mutant) (rec.) activate TLR5 in vivo.Method: Mice WT are injected i.v. with 1ug of Flagellin (rec.) (=WT) or Flagellin (NLRC4 Mutant) (rec.). After 2 hours, levels of CCL20 in the serum are measured by ELISA. As control, WT mice are injected i.v. with 1ug of Flagellin (TLR5 Mutant) (rec.). PBS injected mice are used as negative control.")
![Functional: Recombinant Human, Mouse Flagellin NLRC4 Mutant His Protein [NBP3-18158] - Flagellin (rec.) and Flagellin (NLRC4 Mutant) (rec.)induce CCL20-luciferase promoter through TLR5 in vitro.Method: Caco-2 cells transfected with plasmid encoding CCL20-LUC plasmid are activated for 24h with the indicated concentrations of Flagellin (rec.)and (NLRC4 Mutant) (rec.). As a negative control, Flagellin (TLR5 Mutant) (rec.) is used. Luciferase assays are performed on cell lysates and luminescence is normalized to mock-treated cells.](http://images.novusbio.com/fullsize/Recombinant-Human--Mouse-Flagellin-NLRC4-Mutant-His-Protein-Functional-NBP3-18158-img0001.jpg "Functional: Recombinant Human, Mouse Flagellin NLRC4 Mutant His Protein [NBP3-18158] - Flagellin (rec.) and Flagellin (NLRC4 Mutant) (rec.)induce CCL20-luciferase promoter through TLR5 in vitro.Method: Caco-2 cells transfected with plasmid encoding CCL20-LUC plasmid are activated for 24h with the indicated concentrations of Flagellin (rec.)and (NLRC4 Mutant) (rec.). As a negative control, Flagellin (TLR5 Mutant) (rec.) is used. Luciferase assays are performed on cell lysates and luminescence is normalized to mock-treated cells.")
![ELISA: Flagellin Native Protein [NBP3-18149] - Flagellin activates TLR5 in vivo.Method: Mice WT are injected i.v. with indicated doses of Flagellin. After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10ug of Flagellin, but no expression of CCL20 and IL-6 could be observed (not shown).](http://images.novusbio.com/fullsize/Flagellin-Native-Protein-ELISA-NBP3-18149-img0001.jpg "ELISA: Flagellin Native Protein [NBP3-18149] - Flagellin activates TLR5 in vivo.Method: Mice WT are injected i.v. with indicated doses of Flagellin. After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10ug of Flagellin, but no expression of CCL20 and IL-6 could be observed (not shown).")
![ELISA: Flagellin Native Protein [NBP3-18154] - Flagellin activates TLR5.Method: Caco-2 cells transfected with plasmid encoding CCL20-LUC plasmid were activated for 24h with the indicated concentrations of Flagellin. Luciferase assays were performed on cell lysates and luminescence was normalized to mock-treated cells.](http://images.novusbio.com/fullsize/Flagellin-Native-Protein-ELISA-NBP3-18154-img0001.jpg "ELISA: Flagellin Native Protein [NBP3-18154] - Flagellin activates TLR5.Method: Caco-2 cells transfected with plasmid encoding CCL20-LUC plasmid were activated for 24h with the indicated concentrations of Flagellin. Luciferase assays were performed on cell lysates and luminescence was normalized to mock-treated cells.")