![ELISA: Flagellin Native Protein [NBP3-18149] - Flagellin activates TLR5 in vivo.Method: Mice WT are injected i.v. with indicated doses of Flagellin. After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10ug of Flagellin, but no expression of CCL20 and IL-6 could be observed (not shown).](http://images.novusbio.com/fullsize/Flagellin-Native-Protein-ELISA-NBP3-18149-img0001.jpg "ELISA: Flagellin Native Protein [NBP3-18149] - Flagellin activates TLR5 in vivo.Method: Mice WT are injected i.v. with indicated doses of Flagellin. After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10ug of Flagellin, but no expression of CCL20 and IL-6 could be observed (not shown).")
| Reactivity | BaSpecies Glossary |
| Applications | ELISA, Bioactivity |
| Concentration | LYOPH |
| Description | Source: Salmonella typhimurium strain 14028 Uniprot ID: P06179 |
| Specificity | Binds to human and mouse TLR5 (Toll-like receptor 5). |
| Details of Functionality | Binds to human & mouse TLR5. Activation of TLR5 in human epithelial cell assays based on NF-kB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 & matrilysin (MMP-7). Maturation of dendritic cells in in vitro & in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous & intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (high Purity) at 5-50ng/ml & in vivo at a starting concentration of 0.1-1ug /mouse. |
| Protein/Peptide Type | Native Protein |
| Purity | >95% pure by SDS-PAGE |
| Endotoxin Note | <0.01EU/ug purified protein (LAL test; Lonza). |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | >95% pure by SDS-PAGE |
| Reconstitution Instructions | Reconstitute with 100 ul sterile water (0.1mg/ml after reconstitution) |
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