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107 Results for "fgf r2"
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AF3285

FGFR1-4 [p Tyr653, p Tyr654, p Tyr654] Antibody [Unconjugated]

(8 Publications)
50 μg
AF3285
Western blot shows lysates of KATO-III human gastric carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recom-binant Human FGF acidic (Catalog # 232-FA) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-FGF R1-4 (Y653/Y654) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3285), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-FGF R1-4 (Y653/Y654) at approximately 120 to 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, ICC/IF
Applications: WB, ICC/IF
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF3285-SP
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MAB2331

FGF basic/FGF2/bFGF Antibody (10043R) [Unconjugated]

100 μg
MAB2331
Recombinant Human FGF basic/FGF2/bFGF protein was serially diluted 2-fold and captured by Mouse Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody(Catalog # MAB233R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody (Catalog # MAB2331) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB2331-SP
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MAB233R

FGF basic/FGF2/bFGF Antibody (10060R) [Unconjugated]

100 μg
Hu
Species Glossary
Reactivity: Hu
ELISA(Cap)
Applications: ELISA(Cap)
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB233R-SP
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MAB6841

FGFR2 alpha Antibody (98706) [Unconjugated]

(5 Publications)
100 μg
Hu
Species Glossary
Reactivity: Hu
WB
Applications: WB
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB6841-SP
Carrier Free MAB6841-500
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DYC684-2

Phospho-FGFR2 alpha DuoSet IC ELISA, 2 Plate

(2 Publications)
1 Kit (for 2 Plates)
DYC684-2
Lysates prepared from the human stomach cancer cell line (KatoIII) were diluted in series and analyzed by (A) IP-Western blot and (B) this DuoSet IC ELISA. IPs were performed using an anti-FGF R2 alpha monoclonal antibody and goat anti-mouse agarose. Immunoblots were incubated with a biotinylated anti-FGF R2 alpha monoclonal antibody. Bands were visualized with Streptavidin-HRP (Catalog # DY998) followed by chemiluminescent detection using WesternGlo Chemiluminescent Detection Substrate (Catalog # AR004). Human FGF R2 alpha can be detected by the Human Total-FGF R2 alpha DuoSet IC ELISA by using approximately 20 to 40 times less lysate than is needed for a conventional IP-Western blot.
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
Conjugate Catalog # Availability Size Price
Biotin DYC684E
Biotin DYC684-5
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MAB11119

FGFR2 alpha Antibody (1057909) [Unconjugated]

100 μg
MAB11119
FGFR2 alpha was detected in immersion fixed HT‑29 Human Colon Adenocarcinoma Cell Line (Positive) and absent in HEL 92.1.7 Human Erythroleukemic Cell Line (Negative) using Mouse Anti-Human FGFR2 alpha Monoclonal Antibody (Catalog # MAB11119) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Hu
Species Glossary
Reactivity: Hu
Flow, ICC/IF
Applications: Flow, ICC/IF
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB11119-SP
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663-FR

Recombinant Human FGFR2 alpha (IIIb) Fc Chimera Protein, CF

(2 Publications)
50 μg
Hu
Species Glossary
Reactivity: Hu
Bioactivity
Applications: Bioactivity
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AVI712

Recombinant Human FGFR2 alpha (IIIc) Fc Avi-tag Protein, CF

50 μg
AVI712
In a Human FGF acidic/FGF1 antibody (AF232) coated plate, in the presence of 50.0 ng/mL of Recombinant Human FGF acidic/FGF1 (232-FA), Biotinylated Recombinant Human FGFR2 alpha (IIIc) Fc Avi-tag Protein (Catalog # AVI712) binds with an ED50 of 0.0750‑0.450 µg/mL.
Hu
Species Glossary
Reactivity: Hu
Bioactivity
Applications: Bioactivity
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11119-FR

Recombinant Human FGFR2 alpha (IIIc) His-tag Protein, CF

50 μg
11119-FR
In a Human FGF acidic/FGF1 antibody (AF232) coated plate, in the presence of 50.0 ng/mL of Recombinant Human FGF acidic/FGF1 (232-FA), Human FGFR2 alpha (IIIc) His-tag Protein (Catalog # 11119-FR) binds with an ED50 of 0.400‑2.40 µg/mL.
Hu
Species Glossary
Reactivity: Hu
Bioactivity
Applications: Bioactivity
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AVI663

Recombinant Human FGFR2 alpha (IIIb) Fc Avi-tag Protein, CF

50 μg
AVI663
Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human FGFR2 alpha (IIIb) Fc Chimera Avi-tag Protein (Catalog # AVI663) binds Recombinant Human FGF acidic/FGF1 (aa 16-155) Protein (232-FA) with an ED50 of 0.0500‑0.500 μg/mL
Hu
Species Glossary
Reactivity: Hu
Bioactivity
Applications: Bioactivity
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MAB111191

FGFR2 alpha Antibody (1057941) [Unconjugated]

100 μg
MAB111191
FGFR2 alpha was detected in immersion fixed paraffin-embedded sections of Skin using Mouse Anti-Human FGFR2 alpha Monoclonal Antibody (Catalog # MAB111191) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Hu
Species Glossary
Reactivity: Hu
IHC
Applications: IHC
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB111191-SP
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AVI11119

Recombinant Human FGFR2 alpha (IIIc) His Avi-tag Protein, CF

50 μg
AVI11119
Measured by its binding ability in a functional ELISA. When Recombinant Human FGF acidic/FGF1 (aa 16-155) Protein (232-FA) is present at 50.0 ng/mL (100 μL/well), Biotinylated Recombinant Human FGFR2 alpha (IIIc) His-tag Avi-tag binds with an ED50 of 0.300-3.00 μg/mL.
Hu
Species Glossary
Reactivity: Hu
Bioactivity
Applications: Bioactivity
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