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![Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with conjugated to DyLight 488 (NBP1-47254G) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-47254-img0012.jpg "Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with conjugated to DyLight 488 (NBP1-47254G) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: ERR alpha/NR3B1 Antibody [NBP1-47254] - The protein expression levels in QF muscle. Representative Western blot images. n = 14-15 animals/group. *=vs. LFsed (P 0.1). Black bars = sedentary, grey bars = running. Image collected and cropped by CiteAb from the following publication (//nutritionandmetabolism.biomedcentral.com/articles/10.1186/1743-7075-9-53), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Western-Blot-NBP1-47254-img0011.jpg)
![Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha/ESRRA protein in a section of normal human kidney using 5 ug/ml concentration of Estrogen Related Receptor alpha antibody. Some cells of tubules/ducts in the tested renal tissue depicted intense nuclear staining with an overall weak/diffused cytoplasmic staining. (10X Magnification)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunohistochemistry-Paraffin-NBP1-47254-img0010.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody [NBP1-47254] - Analysis of ERR alpha in A431 cell lysates](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Western-Blot-NBP1-47254-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - ERR alpha antibody was tested in HeLa cells at a 1:100 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-47254-img0007.jpg)
![Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha / ESRRA protein in a section of normal skin of human using 5 ug/ml concentration of Estrogen Related Receptor alpha antibody. The different cell types in the cellular epidermis as well as the blood vessels in the dermal layer showed positivity for ERR alpha, and the staining was found to be more intense in the nuclei compared to cytoplasm. (10X Magnification)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunohistochemistry-Paraffin-NBP1-47254-img0009.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody [NBP2-16380] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Western-Blot-NBP2-16380-img0002.jpg "Western Blot: ERR alpha/NR3B1 Antibody [NBP2-16380] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:2000.")
![Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP2-16380] - Human intestine. ERR alpha stained by ERR alpha antibody [N1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunohistochemistry-Paraffin-NBP2-16380-img0006.jpg)
![Chromatin Immunoprecipitation: ERR alpha/NR3B1 Antibody [NBP2-16380] - Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 ug of either control rabbit IgG or anti-ERR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter or GAPDH promoter.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Chromatin-Immunoprecipitation-NBP2-16380-img0003.jpg)
![Immunoprecipitation: ERR alpha/NR3B1 Antibody [NBP2-16380] - IP Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of ERR alpha protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of ERR alpha by 2.5 ug of ERR alpha antibody 10% SDS-PAGE The immunoprecipitated ERR alpha protein was detected by ERR alpha antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary eagent.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunoprecipitation-NBP2-16380-img0005.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-Western-Blot-NBP2-61768-img0004.jpg "Western Blot: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-Western-Blot-NBP2-61768-img0003.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis using ESRRA mouse mAb against C6 (1) cell lysate.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-Western-Blot-NBP2-61768-img0005.jpg)
![ELISA: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-ELISA-NBP2-61768-img0001.jpg)
![ELISA: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis of Hela cells using ESRRA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-ELISA-NBP2-61768-img0002.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-1A6E11-Western-Blot-NBP2-61769-img0003.jpg "Western Blot: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)")
![Flow Cytometry: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Analysis of Hela cells using ESRRA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-1A6E11-Flow-Cytometry-NBP2-61769-img0002.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-1A6E11-Western-Blot-NBP2-61769-img0004.jpg)
![ELISA: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-1A6E11-ELISA-NBP2-61769-img0001.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody [NBP2-56812] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Western-Blot-NBP2-56812-img0002.jpg "Western Blot: ERR alpha/NR3B1 Antibody [NBP2-56812] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP2-56812] - Staining of human cell line MCF7 shows localization to nucleus, nucleoli fibrillar center, microtubules & cytokinetic bridge.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56812-img0001.jpg)
![SDS-Page: Recombinant Human ERR alpha/NR3B1 Protein [H00002101-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-ERR-alpha-NR3B1-Protein-SDS-Page-H00002101-P01-img0002.jpg "SDS-Page: Recombinant Human ERR alpha/NR3B1 Protein [H00002101-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![SDS-Page: Recombinant Human ERR alpha/NR3B1 Protein [H00002101-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00002101-Q01-1.jpg "SDS-Page: Recombinant Human ERR alpha/NR3B1 Protein [H00002101-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: ERR alpha/NR3B1 Antibody (2R10S3) [NBP3-16349] - Western blot analysis of extracts of various cell lines, using ERR alpha/NR3B1 Rabbit mAb (NBP3-16349) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-2R10S3-Western-Blot-NBP3-16349-img0001.jpg "Western Blot: ERR alpha/NR3B1 Antibody (2R10S3) [NBP3-16349] - Western blot analysis of extracts of various cell lines, using ERR alpha/NR3B1 Rabbit mAb (NBP3-16349) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP3-17645] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunohistochemistry-Paraffin-NBP3-17645-img0001.jpg "Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP3-17645] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.")
![ELISA: Human ERR alpha/NR3B1 ELISA Kit (Colorimetric) [NBP3-31550] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31550_human-err-alpha-nr3b1-elisa-kit-colorimetric-206202415322773.png "ELISA: Human ERR alpha/NR3B1 ELISA Kit (Colorimetric) [NBP3-31550] - Standard Curve Reference")