![Western Blot: West Nile Virus Envelope Antibody (GT3029) [NBP3-13574] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with West Nile Virus Envelope protein antibody [GT3029] (NBP3-13574) diluted at 1:1000.](http://images.novusbio.com/fullsize/West-Nile-Virus-Envelope-Antibody-GT3029-Western-Blot-NBP3-13574-img0004.jpg "Western Blot: West Nile Virus Envelope Antibody (GT3029) [NBP3-13574] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with West Nile Virus Envelope protein antibody [GT3029] (NBP3-13574) diluted at 1:1000.")
| Reactivity | V-ViSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | GT3029 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of West Nile Virus Envelope Protein (West Nile virus (strain NY99-IC)). The exact sequence is proprietary. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 54 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: West Nile Virus Envelope Antibody (GT3029) [NBP3-13574] - West Nile Virus Protein E protein antibody [GT3029] detects West Nile Virus Protein E protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: West Nile Virus Protein E protein stained by West Nile Virus Protein E protein antibody [GT3029] (NBP3-13574) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/West-Nile-Virus-Envelope-Antibody-GT3029-Immunocytochemistry-Immunofluorescence-NBP3-13574-img0002.jpg "Immunocytochemistry/Immunofluorescence: West Nile Virus Envelope Antibody (GT3029) [NBP3-13574] - West Nile Virus Protein E protein antibody [GT3029] detects West Nile Virus Protein E protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: West Nile Virus Protein E protein stained by West Nile Virus Protein E protein antibody [GT3029] (NBP3-13574) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar= 10 um.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")