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V5 Epitope Tag Antibody (1036H) [Unconjugated]

Images

 
Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal V5-tagged CBL, N-terminal V5-tagged FSNC4, C-terminal V5-tagged LAMP2B, and C-terminal V5-tagged GALNT2. PVDF membrane ...read more
HEK293 human embryonic kidney cell line transfected with V5-tagged proteins was stained with Rabbit Anti-V5 Epitope Tag Monoclonal Antibody (Catalog # MAB8926, filled histogram) or isotype control antibody (Catalog # ...read more
Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal V5-tagged CBL and C-terminal V5-tagged GALNT2, loaded at 0.2 mg/mL. Specific bands were detected for V5 ...read more

Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications WB, Simple Western, Flow, IP
Clone
1036H
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

V5 Epitope Tag Antibody (1036H) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
V5 epitope tag peptide
Accession # Y138513
Specificity
Detects N-terminal and C-terminal V5 epitope-tagged proteins in Western blots.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunoprecipitation 1-4 ug/100 ug cell lysate
  • Simple Western 1 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
MAB8926 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
Sodium Azide
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for V5 Epitope Tag Antibody (1036H) [Unconjugated]

  • GKPIPNPLLGLDST Epitope Tag
  • Parainfluenza virus 5 V/P
  • Paramyxovirus SV5
  • V5 Epitope Tag

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for V5 Epitope Tag Antibody (MAB8926)(4)

We have publications tested in 3 confirmed species: Human, Mouse, Mesocricetus auratus (Hamster).

We have publications tested in 2 applications: Immunoprecipitation, Western Blot.


Filter By Application
Immunoprecipitation
(2)
Western Blot
(3)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
Mesocricetus auratus (Hamster)
(1)
All Species
Showing Publications 1 - 4 of 4.
Publications using MAB8926 Applications Species
KS Moore, R Moore, DB Fulmer, L Guo, C Gensemer, R Stairley, J Glover, TC Beck, JE Morningsta, R Biggs, R Muhkerjee, A Awgulewits, RA Norris DCHS1, Lix1L, and the Septin Cytoskeleton: Molecular and Developmental Etiology of Mitral Valve Prolapse Journal of cardiovascular development and disease, 2022-02-17;9(2):. 2022-02-17 [PMID: 35200715] (Immunoprecipitation, Human) Immunoprecipitation Human
H Chu, B Hu, X Huang, Y Chai, D Zhou, Y Wang, H Shuai, D Yang, Y Hou, X Zhang, TT Yuen, JP Cai, AJ Zhang, J Zhou, S Yuan, KK To, IH Chan, KY Sit, DC Foo, IY Wong, AT Ng, TT Cheung, SY Law, WK Au, MA Brindley, Z Chen, KH Kok, JF Chan, KY Yuen Host and viral determinants for efficient SARS-CoV-2 infection of the human lung Nature Communications, 2021-01-08;12(1):134. 2021-01-08 [PMID: 33420022] (Western Blot, Mesocricetus auratus (Hamster)) Western Blot Mesocricetus auratus (Hamster)
GT Banks, MCC Guillaumin, I Heise, P Lau, M Yin, N Bourbia, C Aguilar, MR Bowl, C Esapa, LA Brown, S Hasan, E Tagliatti, E Nicholson, RS Bains, S Wells, VV Vyazovskiy, K Volynski, SN Peirson, PM Nolan Forward genetics identifies a novel sleep mutant with sleep state inertia and REM sleep deficits Sci Adv, 2020-08-12;6(33):eabb3567. 2020-08-12 [PMID: 32851175] (Immunoprecipitation, Western Blot, Human) Immunoprecipitation, Western Blot Human
J Zeng, S Dong, Z Luo, X Xie, B Fu, P Li, C Liu, X Yang, Y Chen, X Wang, Z Liu, J Wu, Y Yan, F Wang, JF Chen, J Zhang, G Long, SA Goldman, S Li, Z Zhao, Q Liang The Zika Virus Capsid Disrupts Corticogenesis by Suppressing Dicer Activity and miRNA Biogenesis Cell Stem Cell, 2020-08-06;0(0):. 2020-08-06 [PMID: 32763144] (Western Blot, Human, Mouse) Western Blot Human, Mouse

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Product General Protocols

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WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional V5 Epitope Tag Products

Blogs on V5 Epitope Tag.

V5 Tags are preferable for detection of Exogenous Proteins in Vivo
Expression of transgenes in complex organisms is accompanied by the need for a specific and sensitive detection of a target protein. One familiar approach is the use of a protein-specific antibody. However, antibodies raised against to your protein of...  Read full blog post.

Working with the V5 Tag Antibody
The V5 tag antibody identifies the V5 epitope tag, enabling proteins of interest to be analyzed and studied via ELISA, Western blot and other immunochemical methods. We at Novus Biologicals are one of the leading suppliers of epitope tag reagents, wit...  Read full blog post.

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