UCK Antibody (OTI1A12)

Images

 
Western Blot: UCK Antibody (1A12) [NBP2-02875] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UCK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: UCK Antibody (1A12) [NBP2-02875] Immunofluorescent staining of HeLa cells using anti-UCK mouse monoclonal antibody.
Flow Cytometry: UCK Antibody (1A12) [NBP2-02875] - Analysis of Jurkat cells, using anti-UCK antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: UCK Antibody (1A12) [NBP2-02875] - Analysis of Hela cells, using anti-UCK antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
OTI1A12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.43 mg/ml

Order Details

UCK Antibody (OTI1A12) Summary

Immunogen
Full length human recombinant protein of human UCK1 (NP_113620) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
UCK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
Theoretical MW
31.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.43 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UCK Antibody (OTI1A12)

  • Cytidine monophosphokinase 1
  • EC 2.7.1.48
  • FLJ12255
  • Uridine monophosphokinase 1
  • uridine-cytidine kinase 1
  • URK1UCK 1

Background

Uridine/cytidine kinase-1 (EC 2.7.1.48) is a pyrimidine ribonucleoside kinase that catalyzes the phosphorylation of uridine and cytidine to form uridine monophosphate (UMP) and cytidine monophosphate (CMP) (Van Rompay et al., 2001 [PubMed 11306702]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for UCK Antibody (NBP2-02875) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on UCK

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Bioinformatics

Gene Symbol UCK1
Entrez