Troponin T Type 2 (cardiac) Antibody (CL13382)

Images

 
Staining of human liver shows no positivity in hepatocytes as expected.
Western blot analysis in human cell line RH-30.
Staining of human testis shows no positivity in cells in seminiferous ducts as expected.
Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.
Orthogonal Strategies: Analysis in human heart muscle and testis tissues using NBP3-24556 antibody. Corresponding TNNT2 RNA-seq data are presented for the same tissues.
Staining of human rectum shows no positivity in glandular cells as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
CL13382
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Troponin T Type 2 (cardiac) Antibody (CL13382) Summary

Immunogen
This antibody was designed to target the recombinant protein Troponin T Type 2 (cardiac), with the precise immunogen sequence being withheld for proprietary reasons.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TNNT2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10000 - 1:20000
  • Immunohistochemistry-Paraffin 1:10000 - 1:20000
  • Western Blot 1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Troponin T Type 2 (cardiac) Antibody (CL13382)

  • cardiac
  • CMD1D
  • CMPD2
  • dilated 1D (autosomal dominant)
  • hypertrophic 2
  • MGC3889
  • RCM3
  • TnTc
  • troponin T type 2 (cardiac)
  • troponin T, cardiac muscle

Background

Does not cross-react with skeletal muscle troponin T, cTnI or TnC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Troponin T Type 2 (cardiac) Antibody (NBP3-24556) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TNNT2