| Reactivity | MuSpecies Glossary |
| Applications | WB, ELISA(Det) |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Biotin |
| Concentration | LYOPH |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse TRANCE (R&D Systems, Catalog # 462-TR) Arg72-Asp316 Accession # O35235 |
| Specificity | Detects mouse TRANCE in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) TRANCE, recombinant mouse (rm) RANK, rhCD40 Ligand, and rmOPG is observed. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | TNFSF11 |
| Purity | Antigen Affinity-purified |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | ELISA Capture: Mouse TRANCE/TNFSF11/RANK L Antibody (Catalog # AF462) ELISA Detection: Mouse TRANCE/TNFSF11/RANK L Biotinylated Antibody (Catalog # BAF462) Standard: Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TR) |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Antigen Affinity-purified |
| Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
TRANCE (receptor activator of NF-kappa B ligand [RANK L], also called TNF-related activation-induced cytokines, osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. In the TNF superfamily nomenclature, TRANCE is referred to as TNFSF11. TRANCE was originally identified as an immediate early gene upregulated by T cell receptor stimulation. The murine TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE share 85% amino acid identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of TRANCE include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. RANK is the cell surface signaling receptor of TRANCE. RANK has been shown to undergo receptor clustering during signal transduction. Osteoprotegerin, a soluble member of the TNF receptor family which binds TRANCE, is a naturally occurring decoy receptor that counterbalances the effects of TRANCE.
Secondary Antibodies |
Isotype Controls |
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The diverse functions of RANKL/TRANCE/TNFSF11 RANKL (also known as TNF-related activation-induced cytokine), or receptor activator of nuclear factor-?B ligand, was first discovered as a key player in the RANKL/RANK/OPG osteoclast formation pathway. Osteoclasts are large multinucleate cells t... Read full blog post. |
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