Novus Biologicals products are now on bio-techne.com

TRA-1-81 Antibody (TRA-1-81)

Images

 
Western Blot: TRA-1-81 Antibody (TRA-1-81) [NB100-1833] - Analysis of NB100-1833 expression in NTERA-2 cl.D1 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) [NB100-1833] - TRA-1-81 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA-1-81 Monoclonal Antibody (Catalog ...read more
Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) [NB100-1833] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-1833. Staining of human cancer line PSN1. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clone
TRA-1-81
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

TRA-1-81 Antibody (TRA-1-81) Summary

Immunogen
Human embryonal carcinoma cell line 2102Ep.
Localization
Cell Membrane
Marker
Embryonic Stem Cell Marker, Tumor Vessel Marker
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Purity
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000. Use reported in scientific literature
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
Publications
Read Publications using
NB100-1833 in the following applications:

Reactivity Notes

This antibody recognizes the TRA-1-81 antigen that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. This antibody recognizes antigens that are associated with a pericellular matrix proteoglycan.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
IgM purified

Alternate Names for TRA-1-81 Antibody (TRA-1-81)

  • gp200
  • MGC138240
  • PC
  • PCLP
  • PODXL
  • TRA181
  • TRA-1-81

Background

TRA-1-81 is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRA-1-81 Antibody (NB100-1833)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: FLOW, ICC/IF, WB.


Filter By Application
FLOW
(1)
ICC/IF
(6)
WB
(1)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-1833 Applications Species
Rossbach B, Hildebrand L, El-Ahmad L. Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector. Stem Cell Research. 2016-03-01 [PMID: 28677531] (ICC/IF, Human)

Details:
This citation used the DyLight 650 form of this antibody.
ICC/IF Human
Rossbach B, Hildebrand L, El-Ahmad L. Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector Stem Cell Research 2016-01-01 [PMID: 27638042] (ICC/IF, Human)

Details:
This reference used the DyLight 650 version of NB100-1833.
ICC/IF Human
Rossbach B, Hildebrand L, El-Ahmad L. Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector. Stem Cell Res 2016-06-28 [PMID: 27345993] (ICC/IF, Human) ICC/IF Human
Hildebrand L, Seemann P, Kurtz A et al. Selective cell targeting and lineage tracing of human induced pluripotent stem cells using recombinant avian retroviruses Cell. Mol Life Sci 2015-06-25 [PMID: 26109426] (ICC/IF, Human)

Details:
This publication used the DL650 conjugated form of this antibody (NB100-1833C)
ICC/IF Human
Mizrak SC, Chikhovskaya JV, Sadri-Ardekani H et al. Embryonic stem cell-like cells derived from adult human testis. Hum Reprod :dep354. 2009-01-01 [PMID: 19815622] (ICC/IF, Human) ICC/IF Human
Henderson, JK et al. Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells;20(4):329-37. 2002-01-01 [PMID: 12110702] (FLOW, ICC/IF, Human) FLOW, ICC/IF Human
Badcock, G et al. The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan. Cancer Res;59(18):4715-9. 1999-09-15 [PMID: 10493530] (WB, Human) WB Human

Reviews for TRA-1-81 Antibody (NB100-1833) (0)

There are no reviews for TRA-1-81 Antibody (NB100-1833). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

View specific protocols for TRA-1-81 Antibody (NB100-1833): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRA-1-81 Antibody (NB100-1833). (Showing 1 - 1 of 1 FAQs).

  1. We would like to know the host of the following antibodies, is BALC/c mouse or not?
    • The lab has informed me that these were both produced in BALB/c mice.

Secondary Antibodies

 

Isotype Controls

Additional TRA-1-81 Products

Array NB100-1833

Research Areas for TRA-1-81 Antibody (NB100-1833)

Find related products by research area.

Blogs on TRA-1-81

There are no specific blogs for TRA-1-81, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TRA-1-81 Antibody (TRA-1-81) and receive a gift card or discount.