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Tie-2 Antibody (tek9) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, CyTOF-ready
Clone
tek9
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-18614AF350
Alexa Fluor 405
NBP1-18614AF405
Alexa Fluor 488
NBP1-18614AF488
Alexa Fluor 532
NBP1-18614AF532
Alexa Fluor 594
NBP1-18614AF594
Alexa Fluor 647
NBP1-18614AF647
Alexa Fluor 700
NBP1-18614AF700
Alexa Fluor 750
NBP1-18614AF750
Allophycocyanin
NBP1-18614APC
Allophycocyanin/Cy7
NBP1-18614APCCY7
Biotin
NBP1-18614B
DyLight 350
NBP1-18614UV
DyLight 405
NBP1-18614V
DyLight 488
NBP1-18614G
DyLight 550
NBP1-18614R
DyLight 594
NBP1-18614DL594
DyLight 650
NBP1-18614C
DyLight 680
NBP1-18614FR
DyLight 755
NBP1-18614IR
FITC
NBP1-18614F
HRP
NBP1-18614H
Janelia Fluor 646
NBP1-18614JF646
PE
NBP1-18614PE
PE/Atto594
NBP1-18614PEATT594
PE/Cy5.5
NBP1-18614PECY55
PE/Cy7
NBP1-18614PECY7
PerCP
NBP1-18614PCP
Unconjugated
NBP1-18614
mFluor Violet 450 SE
NBP1-18614MFV450
mFluor Violet 500 SE
NBP1-18614MFV500
mFluor Violet 610 SE
NBP1-18614MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-18614
Azide and BSA Free
NBP1-18614
Azide and BSA Free
NBP1-18614-0.02mg
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Tie-2 Antibody (tek9) [Janelia Fluor® 549] Summary

Immunogen
Recombinant human soluble extracellular TIE-2
Specificity
The monoclonal antibody will detect native human TIE-2/tek in ELISA experiments and on the surface of different human cell types.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TEK
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Sandwich ELISA
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Tie-2 Antibody (tek9) [Janelia Fluor® 549]

  • angiopoietin-1 receptor
  • CD202b antigen
  • CD202b
  • EC 2.7.10
  • EC 2.7.10.1
  • hTIE2
  • p140 TEK
  • soluble TIE2 variant 1
  • soluble TIE2 variant 2
  • TEK tyrosine kinase, endothelial
  • TEK
  • Tie2
  • Tie-2
  • TIE2CD202b
  • Tunica interna endothelial cell kinase
  • Tyrosine-protein kinase receptor TEK
  • Tyrosine-protein kinase receptor TIE-2
  • venous malformations, multiple cutaneous and mucosal
  • VMCM
  • VMCM1

Background

TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin like loops separated by three epidermal growth factor like repeats that are connected to three fibronectin type III like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell smooth muscle cell communication in venous morphogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Tie-2 Antibody (NBP1-18614JF549) (0)

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Secondary Antibodies

 

Isotype Controls

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Product PDFs

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Calculators

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Bioinformatics

Gene Symbol TEK