Recombinant Human TFIIS His Protein Summary
Description |
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids1-299 of Human TFIIS Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMMGKEEE IARIARRLDK MVTKKSAEGA MDLLRELKAM PITLHLLQST RVGMSVNALR KQSSDEEVIA LAKSLIKSWK KLLDASDAKA RERGRGMPLP TSSRDASEAP DPSRKRPELP RAPSTPRITT FPPVPVTCDA VRNKCREMLT AALQTDHDHV AIGADCERLS AQIEECIFRD VGNTDMKYKN RVRSRISNLK DAKNPDLRRN VLCGAITPQQ IAVMTSEEMA SDELKEIRKA MTKEAIREHQ MARTGGTQTD LFTCGKCRKK NCTYTQVQTR SSDEPMTTFV VCNECGNRWK FC |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
TCEA2 |
Purity |
>85%, by SDS-PAGE |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
36 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 30% glycerol, 0.25M NaCl |
Preservative |
No Preservative |
Concentration |
0.25 mg/ml |
Purity |
>85%, by SDS-PAGE |
Alternate Names for Recombinant Human TFIIS His Protein
Background
TCEA2 is found in the nucleus, where it functions as an SII class transcription elongation factor. Elongation factors in this class are responsible for releasing RNA polymerase II ternary complexes from transcriptional arrest at template-encoded arresting sites. The protein has been shown to interact with general transcription factor IIB, a basal transcription factor. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human TCEA2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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