Talin1 Antibody (93E12) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP
Clone
93E12
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

Talin1 Antibody (93E12) [Janelia Fluor® 549] Summary

Immunogen
Recombinant protein fragment
Specificity
Does not cross react with Talin2.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TLN1
Purity
Protein A purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined. Positive control(s): Immunoblot: Partial purified PVM, Partial purified BRS virus, Gradient-purified RSN-2 virus (5ug/lane); ICC/IF - RSN-2 infected BSC-1 cells.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Talin1 Antibody (93E12) [Janelia Fluor® 549]

  • ILWEQ
  • KIAA1027
  • talin 1
  • Talin1
  • talin-1
  • TLN
  • TLN1

Background

Talin 1 encodes a cytoskeletal protein which is concentrated in areas of cell-substratum and cell-cell contacts. This protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Research Areas for Talin1 Antibody (NBP2-50319JF549)

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Blogs on Talin1

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Bioinformatics

Gene Symbol TLN1