| Reactivity | HuSpecies Glossary |
| Applications | Enzyme Activity |
| Format | Carrier-Free |
| Details of Functionality | Measured by the hydrolysis of pyroglutamyl-AMC. The specific activity is >9,500 pmol/min/μg, as measured under the described conditions. |
| Source | E. coli-derived human PGPEP-1 protein Met1-His209, with a C-terminal 6-His tag |
| Accession # | |
| N-terminal Sequence | Met1 |
| Protein/Peptide Type | Recombinant Enzymes |
| Gene | PGPEP1 |
| Purity | >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane |
| Endotoxin Note | <1.0 EU per 1 μg of the protein by the LAL method. |
| Dilutions |
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| Theoretical MW | 24 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| SDS-PAGE | 24-26 kDa, reducing conditions |
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| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and DTT. |
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| Purity | >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane |
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| Assay Procedure |
1. Dilute rhPGPEP-1 to 0.1 ng/μL in Assay Buffer.
*Adjusted for Substrate Blank
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