pSIVA Apoptosis Detection Flow Cytometry Kit Summary
| Description |
Polarity Sensitive Indicator of Viability (pSIVA (Annexin XII)) is an Annexin based, polarity sensitive probe for the spatiotemporal or kinetic analysis of apoptosis and other forms of cell death. pSIVA (Annexin XII) binding is reversible enabling researchers, for the first time, to detect transient PS exposure which is associated with normal physiological processes as well as with reversible or rescuable apoptosis cell death events. pSIVA (Annexin XII) is conjugated to IANBD, a polarity sensitive dye that fluoresces only when pSIVA is bound to the cell membrane. pSIVA's membrane-bound dependent fluorescence and reversible binding properties are a technological leap for detecting PS exposure and offer additional information on the apoptosis pathway and cell survival compared to Annexin V conjugates. Annexin V binding is nonreversible. |
| Kit Type |
Flow Cytometry Kit |
Applications/Dilutions
| Dilutions |
- Flow (Cell Surface)
- Flow Cytometry 1ul/1 million cells
|
| Application Notes |
Please see PDF manual attached for specific usage information. Use in Flow cell surface reported in scientific literature (PMID 24804954) |
| Reviewed Applications |
Read 1 Review rated 4 using
NBP2-29611 in the following applications:
|
|
| Publications |
Read Publications using
NBP2-29611 in the following applications:
|
|
Reactivity Notes
Human and Mouse reactivity reported in scientific literature (PMID: 24804954)
Packaging, Storage & Formulations
Kit Components
|
Components
|
- 10X Binding Buffer (5 mL or 2 mL)
- 10X PBS (20 mL or 5 mL)
- Propidium Iodide Staining Solution (500 uL or 125 uL)
- pSIVA-IANBD (500 uL or 125 uL)
|
Notes
pSIVA is protected under patent number: 8,541,549
Alternate Names for pSIVA Apoptosis Detection Flow Cytometry Kit
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
Product General Protocols
View specific protocols for pSIVA Apoptosis Detection Kit (NBP2-29611):
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for pSIVA Apoptosis Detection Kit (NBP2-29611). (Showing 1 - 2 of 2 FAQs).
-
Our end user is working on tissues (epithelium of imaginal disk from drosophila leg). The tissues are kept growing on a culture medium and they manage to follow apoptosis properly with orange acrylin but not with annexin V because of background. Did you ever test any of your pSIVA apoptosis kits (NBP2-29382, NBP2-29611) on tissue culture instead of cell culture ? Is there a chance the proble will work on such an application ? Would you be able to provide a sample for this application ? As for the other samples, I'm still waiting on customer feedback and I'll keep you posted.
- Yes, pSIVA has been used in tissue applications as denoted on the publication link. Specifically, I would encourage the research to look at the following publications from the link: 1. Diurnal, localized exposure of phosphatidylserine by rod outer segment tips in wild-type but not Itgb5-/- or Mfge8-/- mouse retina. Ruggiero L, MP Connor, J Chen, R Langen, SC Finnemann. PNAS 109:8145-4148 (2012). Live tissue imaging (mouse retina): Figs 4, 5. S4. pSIVA-IANBD was added to dissected live mouse retina and shown to label the tips of photoreceptor outer segments (POS). > The results suggested that phosphatidylserine (PS) exposure is specific to the POS surface. Furthermore, enhanced PS exposure preceded rod shedding and phagocytosis, suggesting that surface PS exposure promotes these processes. 2. Diurnal photoreceptor outer segment shedding: contributions of the RPE. Finnemann SC, L Ruggiero, MP Connor. Invest Opthalmol Vis Sci 53:4327 (2012). Live Imaging: mouse retina. pSIVA was used quantify the surface exposure of phosphotidylserine on the distal rod photoreceptor outer segments. 3. Characterization of cell cycle modifications induced by electric pulses in human corneal endothelium. Thi MH, Z He, N Campolmi, S Piselli, P Gain, M Peoc'H, JM Dumolllard, S Acquart, O Garraud, G Thuret. Acta Opthalmologica 90:s249 (2012). Live tissue imaging (human cornea organ cultures): pSIVA-IANBD was used to assess cell death following gene electrotransfer into corneal endothelial cells. > 3. Engineering a polarity-sensitive biosensor for time-lapse imaging of apoptotic processess and degeneration. Kim YE, J Chen, JR Chan, R Langen. Nature Methods 7:67-73 (2010a). Real-time live-cell imaging and time-lapse microscopy of apoptosis: Fig 2 (COS-7 cells), Fig 3 (rat neuronal degeneration), Fig 4 (rat axonal degeneration), Fig 5 (rescue of rat neuronal degeneration as visualized by pSIVA. (this was also used in a rat nerve injury tissue model) Please watch the pSIVA webinar on our website for additional information about pSIVA. The webinar is referred to March 25, 2014: Real-Time Tracking of Cell Death Webinar. We would certainly be pleased to offer any of your end user research customers a sample who is willing to provide a short explanation about what they are planning to do, their timeframe, and provide feedback about how it worked for them. For any samples, please specify if you
-
Could you specify the composition of the binding buffer in the pSIVA-INABD Apoptosis/Viability Flow Kit (Cat No:NBP2-29611), because my binding buffer is often exhausted long time before the pSIVA-IANBD and propidium iodide is used up? I assume that it is a standard buffer with additional CaCl2 (like I am preparing it for micrsocopy time lapse studies with the microcopy kit (Cat No: NBP2-29382)).
- Thank you for your inquiry about pSIVA and for using our products. Here is the requested recipe: 10X Binding Buffer: 100mM HEPES pH 7.4, 1.4M NaCl, 25mM CaCl2
Additional pSIVA Apoptosis Detection Products
Blogs on pSIVA Apoptosis Detection
![Flow Cytometry: Polarity Sensitive Indicator of Viability Apoptosis Kit [IANBD] [NBP2-29611] - pSIVA-IANBD(TM) + PI shows progressive population staining as cells move from healthy towards death.](http://images.novusbio.com/fullsize/Polarity-Sensitive-Indicator-of-Viability-Apoptosis-Kit-[IANBD]-Flow-Cytometry-NBP2-29611-img0002.jpg "Flow Cytometry: Polarity Sensitive Indicator of Viability Apoptosis Kit [IANBD] [NBP2-29611] - pSIVA-IANBD(TM) + PI shows progressive population staining as cells move from healthy towards death.")
![Flow Cytometry: Polarity Sensitive Indicator of Viability Apoptosis Kit [IANBD] [NBP2-29611] - pSIVA-IANBD(TM) shows progressive population staining as cells move from healthy towards death.](http://images.novusbio.com/fullsize/Polarity-Sensitive-Indicator-of-Viability-Apoptosis-Kit-[IANBD]-Flow-Cytometry-NBP2-29611-img0001.jpg "Flow Cytometry: Polarity Sensitive Indicator of Viability Apoptosis Kit [IANBD] [NBP2-29611] - pSIVA-IANBD(TM) shows progressive population staining as cells move from healthy towards death.")
