Rat Prokineticin 2 ELISA Kit (Colorimetric) Summary
Specificity |
This kit recognizes Rat Prokineticin 2 in samples. No significant cross-reactivity or interference between Rat Prokineticin 2 and analogues was observed. |
Standard Curve Range |
0.31 - 20 ng/mL |
Sensitivity |
0.19 ng/mL |
Assay Type |
Sandwich-ELISA |
Inter-Assay |
%CV < 5.11 |
Intra-Assay |
%CV < 5.66 |
Spike Recovery |
92 - 108% |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
PROK2 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
|
Alternate Names for Rat Prokineticin 2 ELISA Kit (Colorimetric)
Background
Prokineticin 2 is known to regulate many different biological functions, including neurogenesis, smooth muscle contractility, angiogenesis and circadian rhythm. In serving the latter role, prokineticin 2 functions as an output molecule from the suprachiasmatic nucleus (SCN) of the hypothalamus, that transmits behavioral rhythms, but may also function locally within the SCN to synchronize output. Prokineticin 2 expression is induced by CLOCK and BMAL1 heterodimers and light, and is inhibited by period genes (PER1, PER2 and PER3) and cryptochrome genes (CRY1 and CRY2). Expression is reported in the SCN and among a few other discrete brain areas, including the islands of Calleja, media l preoptic area of the hypothalamus and the shell of the nucleus accumbens as well as in the testis, prostate and, at lower levels, in the small intestine.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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