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Recombinant Mouse PRMT1 His-MBP Protein

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SDS-Page: PRMT1 Protein [NBC1-18445] - PRMT1 isoform2, 84 kDa (750 aa) with a purity of 90% by SDS - PAGE

Product Details

Summary
Reactivity MuSpecies Glossary
Applications PAGE, Enzyme Activity
Concentration
1 mg/ml

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Recombinant Mouse PRMT1 His-MBP Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-MBP tag and corresponding to the amino acids 1-353 of mouse PRMT1

Source: E.coli

Amino Acid Sequence: MHHHHHHMKI EEGKLVIWIN GDKGYNGLAE VGKKFEKDTG IKVTVEHPDK LEEKFPQVAA TGDGPDIIFW AHDRFGGYAQ SGLLAEITPD KAFQDKLYPF TWDAVRYNGK LIAYPIAVEA LSLIYNKDLL PNPPKTWEEI PALDKELKAK GKSALMFNLQ EPYFTWPLIA ADGGYAFKYE NGKYDIKDVG VDNAGAKAGL TFLVDLIKNK HMNADTDYSI AEAAFNKGET AMTINGPWAW SNIDTSKVNY GVTVLPTFKG QPSKPFVGVL SAGINAASPN KELAKEFLEN YLLTDEGLEA VNKDKPLGAV ALKSYEEELA KDPRIAATME NAQKGEIMPN IPQMSAFWYA VRTAVINAAS GRQTVDEALK DAQTNSSSNN NNNNNNNNLG IEGRGSHMAA AEAANCIMEV SCGQAESSEK PNAEDMTSKD YYFDSYAHFG IHEEMLKDEV RTLTYRNSMF HNRHLFKDKV VLDVGSGTGI LCMFAAKAGA RKVIGIECSS ISDYAVKIVK ANKLDHVVTI IKGKVEEVEL PVEKVDIIIS EWMGYCLFYE SMLNTVLHAR DKWLAPDGLI FPDRATLYVT AIEDRQYKDY KIHWWENVYG FDMSCIKDVA IKEPLVDVVD PKQLVTNACL IKEVDIYTVK VEDLTFTSPF CLQVKRNDYV HALVAYFNIE FTRCHKRTGF STSPESPYTH WKQTVFYMED YLTVKTGEEI FGTIGMRPNA KNNRDLDFTI DLDFKGQLCE LSCSTDYRMR

Details of Functionality
Specific activity is > 30 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
PRMT1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • Enzyme Activity
  • SDS-Page
Theoretical MW
84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
40mM Tris-HCl buffer (pH 8.0), 100 mM NaCl, 4mM MgCl2, 2 mM DTT, 40% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Mouse PRMT1 His-MBP Protein

  • ANM1
  • EC 2.1.1
  • EC 2.1.1.-
  • EC 2.1.1.125
  • EC 2.1.1.77
  • HCP1
  • Histone-arginine N-methyltransferase PRMT1
  • HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2
  • HMT1 hnRNP methyltransferase-like 2 (S. cerevisiae)
  • HMT1
  • HMT2
  • HRMT1L2
  • HRMT1L2HMT1 hnRNP methyltransferase-like 2
  • Interferon receptor 1-bound protein 4
  • IR1B4
  • IR1B4protein arginine N-methyltransferase 1
  • PRMT1
  • protein arginine methyltransferase 1

Background

Protein arginine N-methyltransferase 1(PRMT1) is a type I methyltransferase that transfers a methyl group from S-adenosylmethionine to guanidino nitrogens of arginine residues to form monomethylarginine and asymmetric dimethylarginine. Functions of type I arginine methylation in proteins may include regulation of transcription, modulation of the affinity of nucleic acid-binding proteins, regulation of interferon signaling pathways, and targeting of nuclear proteins. Mouse PRMT1(AAF37293) shares 99.9% sequence identity with the human proteins(NP_938074). Recombinant mouse PRMT1, fused to His-MBP tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol PRMT1