![Western Blot: PDE5A Antibody [NB300-957] - HEK293 overexpressing PDE5A and probed with (mock transfection in first lane).](http://images.novusbio.com/fullsize/PDE5A-Antibody-Western-Blot-NB300-957-img0006.jpg "Western Blot: PDE5A Antibody [NB300-957] - HEK293 overexpressing PDE5A and probed with (mock transfection in first lane).")
| Reactivity | Hu, Rt, Bv, CaSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-LINGESGQAKRN corresponding to C-Terminus according to NP_001074.2, NP_246273.2, NP_236914.2, NP_237223. |
| Specificity | This antibody is expected to recognise reported isoforms NP_001074.2, NP_236914.2 and NP_246273.2. |
| Predicted Species | Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | PDE5A |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Approx. 100 kDa band observed in human lung lysates (calculated MW of 100 kDa band according to isoform 1, NP_001074.2). In transfected HEK293 transiently expressing PDE5A isoform 2 a band of approx. 95 kDa is observed. This band is not observed in the non-transfected HEK293 (calculated MW of 94.8 kDa band according to isoform 2, NP_236914.2). |
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| Theoretical MW | 100 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB300-957 | Applications | Species |
|---|---|---|
| Morelli A, Filippi S, Mancina R et al. Androgens regulate phosphodiesterase type 5 expression and functional activity in corpora cavernosa. Endocrinology 2004-05-01 [PMID: 14764637] |
Secondary Antibodies |
Isotype Controls |
Research Areas for PDE5A Antibody (NB300-957)Find related products by research area.
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| Gene Symbol | PDE5A |
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| Uniprot |
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![Western Blot: PDE5A Antibody [NB300-957] - (0.3ug/ml) of human lung lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/PDE5A-Antibody-Western-Blot-NB300-957-img0005.jpg "Western Blot: PDE5A Antibody [NB300-957] - (0.3ug/ml) of human lung lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
