![Western Blot: Nogo Receptor/NgR Antibody [NB100-1536] - (0.3ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Nogo-Receptor-NgR-Antibody-Western-Blot-NB100-1536-img0002.jpg "Western Blot: Nogo Receptor/NgR Antibody [NB100-1536] - (0.3ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Rt, Po, CaSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-LQALPDDTFRDLGN corresponding to internal region according to NP_075380.1. |
| Epitope | internal region |
| Predicted Species | Mouse (100%), Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | RTN4R |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | WB: Experiments gave bands at approx. 90-100 kDa band and 37+40+45 kDa band in human brain lysate after 0.3ug/ml antibody staining. These bands were successfully blocked by the immunizing peptide and they correspond to earlier findings in scientific literature with different antibodies (Wang et al, J Neurosci. 2002 Jul 1;22(13): 5505-15). This protein has a calculated MW of 50.7 kDa band according to NP_075380.1. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Nogo Receptor/NgR Antibody (NB100-1536)Find related products by research area.
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| Gene Symbol | RTN4R |
| Entrez |
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| Uniprot |
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