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Human Nicotinic Acetylcholine R alpha 3/CHRNA3 ELISA Kit (Colorimetric)

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ELISA: Human Nicotinic Acetylcholine R alpha 3/CHRNA3 ELISA Kit (Colorimetric) [NBP3-27380] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue homogenates and other biological fluids
Standard Curve Range
0.312 - 20 ng/mL (example only; lot dependent)
Sensitivity
0.107 ng/mL (example only; lot dependent)

Order Details

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Human Nicotinic Acetylcholine R alpha 3/CHRNA3 ELISA Kit (Colorimetric) Summary

Description
Assay Length: 4.5 hours
Standard Curve Range
0.312 - 20 ng/mL (example only; lot dependent)
Sensitivity
0.107 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CHRNA3

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Reagent A
  2. Detection Reagent B
  3. Diluent Buffer
  4. Instruction manual
  5. Plate sealer for 96 wells
  6. Pre-coated 96T strip plate
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human Nicotinic Acetylcholine R alpha 3/CHRNA3 ELISA Kit (Colorimetric)

  • cholinergic receptor, nicotinic, alpha 3
  • cholinergic receptor, nicotinic, alpha polypeptide 3
  • CHRNA3
  • LNCR2
  • MGC104879
  • NACHRA3
  • neuronal acetylcholine receptor subunit alpha-3
  • neuronal nicotinic acetylcholine receptor, alpha3 subunit
  • Nicotinic Acetylcholine R alpha 3
  • Nicotinic Acetylcholine Ra3
  • PAOD2

Background

The nicotinic acetylcholine receptor (nAChR) is a ligand gated ion channel that mediates neurotransmission at the neuromuscular junction, autonomic ganglia and at some sites in the central nervous system. Distinct nAChR subtypes exist that can be stimulated by the neurotransmitter acetylcholine, the natural product nicotine, or by synthetic compounds. After binding acetylcholine, Nicotinic Acetylcholine Receptor alpha 3 responds by an extensive change in conformation that affects all subunits and leads to opening of an ion conducting channel across the plasma membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for Nicotinic Acetylcholine R alpha 3/CHRNA3 ELISA Kit (NBP3-27380) (0)

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FAQs for Nicotinic Acetylcholine R alpha 3/CHRNA3 ELISA Kit (NBP3-27380). (Showing 1 - 1 of 1 FAQ).

  1. I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.
    • A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.

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Bioinformatics

Gene Symbol CHRNA3