Reactivity | Hu, Mu, Rt, Po, ChSpecies Glossary |
Applications | Flow, IHC |
Clone | RT-97 + NR-4 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Concentration | 0.2 mg/ml |
Description | 200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47664) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous. |
Immunogen | Triton-X 100 insoluble proteins of rat brain (RT-97); Neurofilaments from porcine spinal cord (NR-4) (Uniprot: P12036) |
Localization | Cytoplasmic |
Marker | Neuronal Marker |
Specificity | This monoclonal antibody reacts with a 200kDa and 68kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament. |
Isotype | IgG1 Kappa/IgG1 Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | NEFH |
Purity | Protein G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
Storage | Store at 4C. |
Buffer | 10 mM PBS with 0.05% BSA |
Preservative | 0.05% Sodium Azide |
Concentration | 0.2 mg/ml |
Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for NF-H Antibody (NBP2-45280)Find related products by research area.
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