Applications/Dilutions
| Dilutions |
- Chromatin Immunoprecipitation (ChIP) 0.5 uM
|
| Application Notes |
This MYOD1 primer is useful for PCR in chromatin precipitation. Amplicon size for this primer set is 112 base pairs. |
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles. |
| Buffer |
10 uM in TE buffer. |
| Preservative |
No Preservative |
Background
Primers are designed to amplify DNA in exon 1 of the human MyoD1 gene locus. MyoD1 is expressed during muscle development and therefore silent in most cell types. MyoD1 is associated with modified histones targeted to repressed genes.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are
guaranteed for 6 months from date of receipt.
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Blogs on MYOD1 primer
![Chromatin Immunoprecipitation: MYOD1 primer [NBP1-71652] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.](http://images.novusbio.com/fullsize/MYOD1-primer-Chromatin-Immunoprecipitation-NBP1-71652-img0003.jpg "Chromatin Immunoprecipitation: MYOD1 primer [NBP1-71652] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.")
![Chromatin Immunoprecipitation: MYOD1 primer [NBP1-71652] - 2 ug of NB21-1072 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/MYOD1-primer-Chromatin-Immunoprecipitation-NBP1-71652-img0004.jpg "Chromatin Immunoprecipitation: MYOD1 primer [NBP1-71652] - 2 ug of NB21-1072 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).")
