By immunoelectrophoresis and ELISA this reacts specifically with lambda light chains common to all mouse immunoglobulins. No was detected against kapp light chain or non-immunoglobulin serum proteins.
Alternate Names for Goat anti-Mouse Lambda Light Chain Secondary Antibody
IGLC
immunoglobulin lambda constant group
Background
All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). This antibody stains only lambda light chain containing cells in human lymphoid tissues.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
Publications for Lambda Light Chain Antibody (NB7550)(4)
We have publications tested in 1 confirmed species: Mouse.
We have publications tested in 2 applications: ELISA, IF/IHC.
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Showing Publications 1 - 4 of 4.
Publications using NB7550
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Hung L et al.. The Monoclonal Antibody Recognized Apoptosis-Associated Protein in PCV2-Infected Peripheral Blood Mononuclear Cells Preprints 2019-11-13 (ELISA, Mouse)
Details: Citation using the HRP format of this antibody.
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![Western Blot: Goat anti-Mouse Lambda Light Chain Secondary Antibody [NB7550] - Western Blot: Goat anti-Mouse Lambda Light Chain Secondary Antibody [HRP] [NB7552] - Goat anti-Mouse lambda Light Chain Antibody [HRP] [NB7552] - This secondary antibody was used for detection of beta-Actin in human pancreatic cancer cell lysates using beta-Actin antibody (Cat# NBP1-47423). Image from verified customer review. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-Lambda-Light-Chain-Secondary-Antibody-Western-Blot-NB7550-img0001.jpg "Western Blot: Goat anti-Mouse Lambda Light Chain Secondary Antibody [NB7550] - Western Blot: Goat anti-Mouse Lambda Light Chain Secondary Antibody [HRP] [NB7552] - Goat anti-Mouse lambda Light Chain Antibody [HRP] [NB7552] - This secondary antibody was used for detection of beta-Actin in human pancreatic cancer cell lysates using beta-Actin antibody (Cat# NBP1-47423). Image from verified customer review. Image using the HRP form of this antibody.")
