Novus Biologicals products are now on bio-techne.com

IL-27 p28/IL-30 Antibody

Images

 
IL-27 p28/IL-30 was detected in perfusion fixed paraffin-embedded sections of mouse thymus using 15 µg/mL Mouse IL-27 p28/ IL-30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1834) overnight at 4 °C. ...read more
Recombinant Mouse IL‑27 (Catalog # 2799-ML) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line), as measured ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

IL-27 p28/IL-30 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-27 p28/IL-30
Phe29-Ser234
Accession # Q8K3I6
Specificity
Detects mouse IL-27 p28/IL-30 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
Il27
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 1-5 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF1834 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-27 p28/IL-30 Antibody

  • IL27 p28
  • IL-27 p28
  • IL27;IL-27;IL27A;IL-27A;IL27p28;IL-27p28;IL30;p28
  • IL-30

Background

 IL-27 p28 subunit (also known as IL-30 and Interleukin-27 subunit alpha) is a 26 kDa glycoprotein. IL-27 is a heterodimeric cytokine consisting of the IL-27 p28 subunit that is an IL-12 p35 related protein and EBI3, which is related to IL-12 p40. The biological effects of IL-27 are mediated by the heteromeric receptor complex gp130 and TCCR/WSX-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-27 p28/IL-30 Antibody (AF1834)(18)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ELISA Capture, ELISA Development, Neutralization, Western Blot.


Filter By Application
ELISA Capture
(1)
ELISA Development
(1)
Neutralization
(13)
Western Blot
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(14)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using AF1834 Applications Species
Mengmeng Zhao, Zihui Zheng, Pingan Zhang, Yao Xu, Jishou Zhang, Shanshan Peng et al. IL-30 protects against sepsis-induced myocardial dysfunction by inhibiting pro-inflammatory macrophage polarization and pyroptosis iScience 2023-09-15 [PMID: 37636037]
C Sorrentino, L D'Antonio, SL Ciummo, C Fieni, L Landuzzi, F Ruzzi, S Vespa, P Lanuti, LV Lotti, PL Lollini, E Di Carlo CRISPR/Cas9-mediated deletion of Interleukin-30 suppresses IGF1 and CXCL5 and boosts SOCS3 reducing prostate cancer growth and mortality Journal of hematology & oncology, 2022-10-13;15(1):145. 2022-10-13 [PMID: 36224639] (Neutralization, Mouse) Neutralization Mouse
LD Faustino, JW Griffith, RA Rahimi, K Nepal, DL Hamilos, JL Cho, BD Medoff, JJ Moon, DAA Vignali, AD Luster Interleukin-33 activates regulatory T cells to suppress innate &amp;gamma&amp;delta T cell responses in the lung Nat Immunol, 2020-09-28;0(0):. 2020-09-28 [PMID: 32989331] (Neutralization, Mouse) Neutralization Mouse
I Mizoguchi, M Ohashi, H Hasegawa, Y Chiba, N Orii, S Inoue, C Kawana, M Xu, K Sudo, K Fujita, M Kuroda, SI Hashimoto, K Matsushima, T Yoshimoto EBV-induced gene 3 augments IL-23R&amp;alpha protein expression through a chaperone calnexin J. Clin. Invest., 2020-11-02;0(0):. 2020-11-02 [PMID: 32809973] (Western Blot, Mouse) Western Blot Mouse
MC Cavé, S Maillard, K Hildenbran, C Mamelonet, MJ Feige, O Devergne Glycosaminoglycans bind human IL-27 and regulate its activity Eur. J. Immunol., 2020-07-02;0(0):. 2020-07-02 [PMID: 32483835] (ELISA Capture, Human) ELISA Capture Human
Anabela Cordeiro-d Interleukin-27 Early Impacts Leishmania infantum Infection in Mice and Correlates with Active Visceral Disease in Humans Front Immunol, 2016-11-04;7(0):478. 2016-11-04 [PMID: 27867384] (Neutralization, Mouse) Neutralization Mouse
Thymic DCs derived IL-27 regulates the final maturation of CD4(+) SP thymocytes Sci Rep, 2016-07-29;6(0):30448. 2016-07-29 [PMID: 27469302] (Neutralization, Mouse) Neutralization Mouse
Type I interferons and microbial metabolites of tryptophan modulate astrocyte activity and central nervous system inflammation via the aryl hydrocarbon receptor Nat Med, 2016-05-09;0(0):. 2016-05-09 [PMID: 27158906] (Neutralization, Mouse) Neutralization Mouse
Poncini C, Ilarregui J, Batalla E, Engels S, Cerliani J, Cucher M, van Kooyk Y, Gonzalez-Cappa S, Rabinovich G Trypanosoma cruzi Infection Imparts a Regulatory Program in Dendritic Cells and T Cells via Galectin-1-Dependent Mechanisms. J Immunol, 2015-08-31;195(7):3311-24. 2015-08-31 [PMID: 26324777] (Neutralization, Mouse) Neutralization Mouse
Dann , Sara M, Le , Christin, Choudhury , Barun K, Liu , Houpu, Saldarriaga , Omar, Hanson , Elaine M, Cong , Yingzi, Eckmann , Lars Attenuation of intestinal inflammation in interleukin-10-deficient mice infected with Citrobacter rodentium. Infect Immun, 2014-02-24;82(5):1949-58. 2014-02-24 [PMID: 24566625] (Neutralization, Mouse) Neutralization Mouse
Show All 18 Publications.

Reviews for IL-27 p28/IL-30 Antibody (AF1834) (0)

There are no reviews for IL-27 p28/IL-30 Antibody (AF1834). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-27 p28/IL-30 Antibody (AF1834) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IL-27 p28/IL-30 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol Il27
Uniprot