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IL-22R alpha 1 Antibody (496514) [Phycoerythrin]

Images

 
Hepa 1-6 mouse hepatoma cell line was stained with Rat Anti-Mouse IL-22 Ra1 PE-conjugated Monoclonal Antibody (Catalog # FAB42941P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
496514
Clonality
Monoclonal
Host
Rat
Conjugate
Phycoerythrin

Order Details

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IL-22R alpha 1 Antibody (496514) [Phycoerythrin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-22 R alpha 1
Thr18-Ala228
Accession # Q80XZ4
Specificity
Detects mouse IL-22 R alpha 1 in direct ELISAs. In direct ELISAs, 25% cross-reactivity with recombinant human (rh) IL‑22 R alpha 1 is observed and no cross-reactivity with rhIL-20 R alpha , recombinant mouse (rm) IL-20 R alpha , rhIL-22BP, or rmIL-22BP is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
IL22RA1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Reviewed Applications
Read 1 Review rated 4
using
FAB42941P in the following application:

Publications
Read Publication using FAB42941P.

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-22R alpha 1 Antibody (496514) [Phycoerythrin]

  • CRF2-9
  • CRF2-9interleukin 22 receptor
  • Cytokine receptor class-II member 9
  • Cytokine receptor family 2 member 9
  • IL-22 R alpha 1
  • IL-22 receptor subunit alpha-1
  • IL22R alpha 1
  • IL22R
  • IL22R1
  • IL22RA1
  • IL-22Ra1
  • IL-22R-alpha-1
  • IL-TIF-R1
  • interleukin 22 receptor, alpha 1
  • interleukin-22 receptor subunit alpha-1
  • zcytoR11

Background

The IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa type I transmembrane glycoprotein that belongs to the type II cytokine receptor family (CRF). IL-22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 330 aa cytoplasmic domain (1). Within the ECD, mouse IL-22 R alpha 1 shares 78%, 78%, and 94% aa sequence identity with canine, human, and rat IL-22 R alpha 1, respectively. It shares 20%‑26% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22, and -24 receptors (2). IL-22 R alpha 1/IL-10 R beta is an IL-22 responsive receptor (3, 4), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (5, 6). In both cases, IL‑22 R alpha 1 functions as the high affinity ligand binding subunit, and subsequent association with IL-10 R beta or IL-20 R beta serves to stabilize the complex (3, 6‑9). IL‑22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but association with IL-10 R beta or IL-20 R beta is required for signal transduction (3, 7). IL-22BP functions as a competitive antagonist by binding IL-22 and preventing its association with IL-22 R alpha 1 (8, 10). Even though it is a receptor for interleukins, IL‑22 R alpha 1 is not expressed on hematopoietic cells (7, 11, 12). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (7, 11‑14). IL‑22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (4, 12, 14, 15). IL‑22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (4, 15).

  1. Tachiiri, A. et al. (2003) Genes Immun. 4:153.
  2. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33. 
  3. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335. 
  4. Boniface, K. et al. (2005) J. Immunol. 174:3695. 
  5. Dumoutier, L. et al. (2001) J. Immunol. 167:3545. 
  6. Wang, M. et al. (2002) J. Biol. Chem. 277:7341. 
  7. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725. 
  8. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
  9. Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099
  10. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
  11. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
  12. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
  13. Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
  14. Wolk, K. et al. (2004) Immunity 21:241.
  15. Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-22R alpha 1 Antibody (FAB42941P)(1)

Review for IL-22R alpha 1 Antibody (FAB42941P) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using FAB42941P:
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(1)
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Images Ratings Applications Species Date Details
Flow Cytometry IL-22R alpha 1 FAB42941P
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4
reviewed by:
Mahesh Raundhal
Flow Mouse 03/13/2018
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Summary

ApplicationFlow Cytometry
Sample TestedMurine Splenocytes (C57B6)
SpeciesMouse
LotX678a9

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Recent Reviews

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Mahesh Raundhal
03/13/2018
Application: Flow
Species: Mouse

Bioinformatics

Gene Symbol IL22RA1
Uniprot