| Reactivity | MuSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF |
| Clone | MG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | HRP |
| Concentration | LYOPH |
| Description | Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. This antibody has been prepared from immunodeficient mouse ascites by protein A chromatography using specific conditions for subclass purification. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG1 and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA. Peroxidase Conjugated Mouse IgG1 was proven by Dot Blot. |
| Specificity | Isotyping assay resulted the material is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA. Peroxidase Conjugated Mouse IgG1 was proven by Dot Blot. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A purified |
| Dilutions |
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| Application Notes | This isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important. NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly). |
| Storage | Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Gentamicin Sulfate |
| Concentration | LYOPH |
| Purity | Protein A purified |
| Reconstitution Instructions | Reconstitute with 100 ul deionized water (or equivalent). |
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