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Mouse IgG1 Isotype Control (MG1) [Alexa Fluor® 750]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, PAGE
Clone
MG1
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 750

Order Details

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Mouse IgG1 Isotype Control (MG1) [Alexa Fluor® 750] Summary

Specificity
Isotyping assay resulted the material is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA. Fluorescein Conjugated Mouse IgG1 was proven by Dot Blot.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Selective Precipitation

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Fluorophore-linked immunosorbent assay
  • Immunocytochemistry/ Immunofluorescence
  • SDS-Page
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Selective Precipitation

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Mouse IgG1 Isotype Control (MG1) [Alexa Fluor® 750]

  • IgG1
  • Igh-4
  • RGD1359539
  • VH7183

Background

IgG is a monomeric immunoglobulin composed of two heavy chains and two light chains. There are four subclasses of the IgG: IgG1, IgG2, IgG3 and IgG4. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin as well as the only isotype that can pass through the placent,a thereby providing protection to the fetus in its first weeks of life before, its own immune system has developed. IgG can bind to several different kinds of pathogens, for example viruses, bacteria and fungi, and it protects the body against them by complement activation (the classic pathway), opsonization for phagocytosis and neutralization of their toxins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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