Human Coronavirus Spike Protein Antibody (11) [Alexa Fluor® 405] Summary
| Immunogen |
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Coronavirus Spike S1+S2 ECD protein. |
| Specificity |
Recognizes the Spike of HCoV-229E. Doesn't recognize the Spike of SARS-CoV-2, SARS-CoV, MERS-CoV, HCoV-NL63, HCoV-HKU1 (isolate N5), HCoV-OC43.
Has cross-reactivity in ELISA and WB with:
HCoV-229E Spike S1+S2 ECD-His
No cross-reactivity in ELISA and WB with:
SARS-CoV-2 Spike S1-His
SARS-CoV-2 Spike S1 NTD-Fc & AVI
SARS-CoV-2 Spike S1 NTD-His & AVI
SARS-CoV-2 Spike RBD-His
SARS-CoV-2 Spike S1+S2 ECD-His
SARS-CoV-2 Spike S2 ECD-His
SARS-CoV Spike S1+S2 ECD-His
MERS-CoV Spike S1+S2 ECD-His
HCoV-OC43 Spike S1+S2 ECD-His
HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-His
HCoV-NL63 Spike S1+S2 ECD-His |
| Isotype |
IgG1 |
| Clonality |
Monoclonal |
| Host |
Mouse |
| Gene |
S |
| Purity |
Protein A purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Optimal dilution of this antibody should be experimentally determined. |
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
| Buffer |
50mM Sodium Borate |
| Preservative |
0.05% Sodium Azide |
| Purity |
Protein A purified |
Notes
Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for Human Coronavirus Spike Protein Antibody (11) [Alexa Fluor® 405]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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FAQs for Human Coronavirus Spike Protein Antibody (NBP3-14653AF405) (0)
Secondary Antibodies
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Isotype Controls
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Additional Human Coronavirus Spike Protein Products
Blogs on Human Coronavirus Spike Protein
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")