Histone H2AX [p Ser139] Antibody (9F3)

Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4). The H2AX variant represents between 2-25% of total H2A (1-3). Human H2AX protein is 143 amino acids in length and has a theoretical molecular weight of 15 kDa (5). As a result of DSBs, H2AX becomes phosphorylated at serine-139 and gamma H2AX is generated as a response to genomic instability (1-4). Gamma H2AX and its phosphorylation can be detected through western blotting and immunofluorescence, serving as a biomarker for DSB frequency (1-4). Ataxia telangiectasia mutated (ATM) is considered a key protein for H2AX phosphorylation (1-4). Upon the phosphorylation and formation of gamma H2AX, many repair proteins become associated including breast cancer 1 protein (BRCA1), NBS1 (Nijmegen breakage syndrome 1)/hMRE11 (human meiotic recombination 11 protein)/ hRAD50 (N/M/R) repair complex, and 53BP1 (p53 binding protein 1) (1-4). Gamma H2AX detection assays are currently utilized for a variety of purposes, from studying DNA repair to drug development and radiation biodosimetry (4).

References

1. Palla, V. V., Karaolanis, G., Katafigiotis, I., Anastasiou, I., Patapis, P., Dimitroulis, D., & Perrea, D. (2017). gamma-H2AX: Can it be established as a classical cancer prognostic factor?. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. https://doi.org/10.1177/1010428317695931

2. Kuo, L. J., & Yang, L. X. (2008). Gamma-H2AX - a novel biomarker for DNA double-strand breaks. In vivo (Athens, Greece).

3. Kinner, A., Wu, W., Staudt, C., & Iliakis, G. (2008). Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin. Nucleic acids research. https://doi.org/10.1093/nar/gkn550

4. Redon, C. E., Weyemi, U., Parekh, P. R., Huang, D., Burrell, A. S., & Bonner, W. M. (2012). gamma-H2AX and other histone post-translational modifications in the clinic. Biochimica et biophysica acta. https://doi.org/10.1016/j.bbagrm.2012.02.021

5. H2AX: Uniprot (P16104)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC, ICC/IF, WB.

Showing Publications 1 - 4 of 4.

Publications using NBP1-19255	Applications	Species
Yang JH, Hayano M, Griffin PT et al. Loss of epigenetic information as a cause of mammalian aging Cell 2023-01-19 [PMID: 36638792] (Mouse) Details: See supplemental figure S1		Mouse
Lee CF, Caudal A, Abell L et al. Targeting NAD+ Metabolism as Interventions for Mitochondrial Disease Sci Rep 2019-02-28 [PMID: 30816177] (WB, Mouse)	WB	Mouse
Yang J, Griffin P, Vera D et al. Erosion of the Epigenetic Landscape and Loss of Cellular Identity as a Cause of Aging in Mammals SSRN Journal 2019-10-03 (ICC/IF, Mouse)	ICC/IF	Mouse
Pal P, Hales K, Hales DB Increased Transient Receptor Potential Melastatin 8 Expression in the development of bladder pain in patients with Interstitial cystitis/Bladder Pain Syndrome Oncotarget 2020-10-10 [PMID: 33088425] (ICC, Human)	ICC	Human

Array NBP1-19255

• Histone H2AX Antibodies
• Histone H2AX Lysate
• Histone H2AX Proteins
• Histone H2AX ELISA