Biological Strategies: Western Blot: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Adiponectin promotes UCP2 expression in hepatic endothelial cells.AKO mice were treated as in Figure 1B. ...read more
Immunocytochemistry/ Immunofluorescence: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Release of structural liver cells into the perfusate after cold ischemia. Representative images of surface ...read more
Immunohistochemistry-Frozen: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Frozen rat liver tissue sections. DAPI (blue), SE1 (green), cytochrome P450 (red). Tissue sections were acetone fixed. ...read more
Flow Cytometry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Representative sequential gating during flow cytometry analysis of rat non-parenchymal liver cells (n = 4). Cells were labeled with ...read more
Western Blot: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Total protein from human, mouse and rat liver was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% ...read more
Immunohistochemistry-Frozen: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Analysis of frozen normal rat liver tissue sections using anti-Hepatic Sinusoidal Endothelial Cells antibody (green). ...read more
Flow Cytometry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Using the FITC direct conjugate Flow Cytometry: Surface staining of Rat Liver sinusoidal endothelial cells with Mouse anti-Rat Hepatic ...read more
Immunocytochemistry/ Immunofluorescence: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Expression of scavenger receptors & immune lectins in rat LSECs & KCs. a. Unscaled heatmaps of normalized ...read more
Flow Cytometry reported in scientific literature (PMID 9428229)
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry 1-5 ug/ml
Immunohistochemistry-Frozen 1-5 ug/ml
Immunohistochemistry-Paraffin 1-5 ug/ml
Western Blot 1-5 ug/ml
Application Notes
In WB, Hepatic Sinusoidal Endothelial Cells antibody (clone SE-1) generates a specific band around 45-50 kDa molecular weight position. For IHC use neutral buffered formalin fixated (perfusion fixation is recommended) paraffin embedded tissues after proteinase K treatment or acetone fixed frozen sections.
Reviewed Applications
Read 3 Reviews rated 3.7 using NB110-68095 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified
Alternate Names for Hepatic Sinusoidal Endothelial Cells Antibody (SE-1)
Hepatic cells
Hepatic Sinusoidal Endothelial Cells
HSE
SE1
Sinusoidal Endothelial Cells
Background
Hepatic sinusoidal endothelial cells (HSEC) plays a key role in various hepatic pathophysiological conditions and are known for their morphological uniqueness - numerous open fenestrations clustered in groups (sieve plates), lack of distinct basal lamina, and abundant vacuolar/vesicular structures. HSECs are distinct from vascular endothelial cells functionally/morphologically and besides providing sinusoidal barrier, they implicates in the balance or distribution of lipids, cholesterol, and vitamin A, and in the uptake of various substances via their specialized endocytotic mechanisms. SE-1 monoclonal antibody has been specifically designed to recognize an antigen expressed in HSEC, but not in other cell types including vascular endothelial cells. Experiments on developing liver have revealed that the expression of SE-1 antigen is possibly regulated via intimate association of HSECs with hepatocytes. Hepatic sinusoidal endothelial cells (SE-1) antibody has been extensively used as one of the most reliable markers for rat HSEC by Western blot and immunohistochemistry analysis and is a useful tool for studying the role of HSE in various hepato-pathophysiological conditions.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for Hepatic Sinusoidal Endothelial Cells Antibody (NB110-68095)(26)
We have publications tested in 3 confirmed species: Human, Mouse, Rat.
We have publications tested in 6 applications: FLOW, ICC/IF, IF/IHC, IHC-P, Immunohistochemistry, WB.
Zhang R, Liu S, Ma Z et al. Effect of Qizhu Granule on Liver Sinusoidal Endothelial Cells Capillarization in Carbon Tetrachloride-Induced Rats Fibrosis. Research Square 2020-09-17 (WB, Rat)
Normal rat liver Acetone fixation SE1 antibody used at 1:500 dilution, incubated at 4C overnight Multiplexed with cytochrome P450 antibody from another vendor
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![Western Blot: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Adiponectin promotes UCP2 expression in hepatic endothelial cells.AKO mice were treated as in Figure 1B. The NPCs were used for further fractionation to collect those enriched with Kupffer (K)- and sinusoidal endothelial cells. The enrichment of the two cell types were confirmed by Western blotting using macrophage marker F4/80 and sinusoidal endothelial marker SE-1, respectively. UCP2 expression was monitored as in Figure 1. After densitometry analysis, the protein ratio of UCP2/beta-actin was calculated and presented as fold changes against Luci Kupffer samples. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0032349), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hepatic-Sinusoidal-Endothelial-Cells-Antibody-SE-1-Western-Blot-NB110-68095-img0009.jpg "Western Blot: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Adiponectin promotes UCP2 expression in hepatic endothelial cells.AKO mice were treated as in Figure 1B. The NPCs were used for further fractionation to collect those enriched with Kupffer (K)- and sinusoidal endothelial cells. The enrichment of the two cell types were confirmed by Western blotting using macrophage marker F4/80 and sinusoidal endothelial marker SE-1, respectively. UCP2 expression was monitored as in Figure 1. After densitometry analysis, the protein ratio of UCP2/beta-actin was calculated and presented as fold changes against Luci Kupffer samples. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0032349), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Release of structural liver cells into the perfusate after cold ischemia. Representative images of surface marker expression of hepatocytes (top), LSEC (middle), and stellate cells (below). Scale bars: 5 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-020-57589-4), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hepatic-Sinusoidal-Endothelial-Cells-Antibody-SE-1-Immunocytochemistry-Immunofluorescence-NB110-68095-img0010.jpg "Immunocytochemistry/Immunofluorescence: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Release of structural liver cells into the perfusate after cold ischemia. Representative images of surface marker expression of hepatocytes (top), LSEC (middle), and stellate cells (below). Scale bars: 5 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-020-57589-4), licensed under a CC-BY license.")
![Immunohistochemistry-Frozen: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Frozen rat liver tissue sections. DAPI (blue), SE1 (green), cytochrome P450 (red). Tissue sections were acetone fixed. SE1 antibody at 1:500, incubated at 4C overnight. Multiplexed with cytochrome P450 from another vendor. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Hepatic-Sinusoidal-Endothelial-Cells-Antibody-SE-1-Immunohistochemistry-Frozen-NB110-68095-img0008.jpg "Immunohistochemistry-Frozen: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Frozen rat liver tissue sections. DAPI (blue), SE1 (green), cytochrome P450 (red). Tissue sections were acetone fixed. SE1 antibody at 1:500, incubated at 4C overnight. Multiplexed with cytochrome P450 from another vendor. IHC-Fr image submitted by a verified customer review.")
![Flow Cytometry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Representative sequential gating during flow cytometry analysis of rat non-parenchymal liver cells (n = 4). Cells were labeled with antibodies to SE-1 (NB110-68095AF488), CD31, and CD45 (NB100-64895PE). LSECs were then identified as SE-1-Alexa488+ cells (e), and the biexponential CD45-PE/CD31-APC of events (f) were used to display and select the CD45+ CD31+ subsets of LSECs. FMOs (used for gating), and single antibody staining controls are shown in Additional file. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33246411/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hepatic-Sinusoidal-Endothelial-Cells-Antibody-SE-1-Flow-Cytometry-NB110-68095-img0011.jpg "Flow Cytometry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Representative sequential gating during flow cytometry analysis of rat non-parenchymal liver cells (n = 4). Cells were labeled with antibodies to SE-1 (NB110-68095AF488), CD31, and CD45 (NB100-64895PE). LSECs were then identified as SE-1-Alexa488+ cells (e), and the biexponential CD45-PE/CD31-APC of events (f) were used to display and select the CD45+ CD31+ subsets of LSECs. FMOs (used for gating), and single antibody staining controls are shown in Additional file. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33246411/) licensed under a CC-BY license.")
![Western Blot: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Total protein from human, mouse and rat liver was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-H.S.E.C in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Hepatic-Sinusoidal-Endothelial-Cells-Antibody-SE-1-Western-Blot-NB110-68095-img0006.jpg "Western Blot: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Total protein from human, mouse and rat liver was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-H.S.E.C in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Frozen: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Analysis of frozen normal rat liver tissue sections using anti-Hepatic Sinusoidal Endothelial Cells antibody (green). Nuclei were counterstained with DAPI (blue). Image from verified customer review.](http://images.novusbio.com/fullsize/Hepatic-Sinusoidal-Endothelial-Cells-Antibody-SE-1-Immunohistochemistry-Frozen-NB110-68095-img0004.jpg "Immunohistochemistry-Frozen: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Analysis of frozen normal rat liver tissue sections using anti-Hepatic Sinusoidal Endothelial Cells antibody (green). Nuclei were counterstained with DAPI (blue). Image from verified customer review.")
![Flow Cytometry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Using the FITC direct conjugate Flow Cytometry: Surface staining of Rat Liver sinusoidal endothelial cells with Mouse anti-Rat Hepatic Sinusoidal Endothelial Cells [FITC] [NB110-68095F] and negative control. Total viable cells were used for analysis. Image courtesy of customer.](http://images.novusbio.com/fullsize/Hepatic-Sinusoidal-Endothelial-Cells-Antibody-SE-1-Flow-Cytometry-NB110-68095-img0007.jpg "Flow Cytometry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Using the FITC direct conjugate Flow Cytometry: Surface staining of Rat Liver sinusoidal endothelial cells with Mouse anti-Rat Hepatic Sinusoidal Endothelial Cells [FITC] [NB110-68095F] and negative control. Total viable cells were used for analysis. Image courtesy of customer.")
![Immunocytochemistry/ Immunofluorescence: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Expression of scavenger receptors & immune lectins in rat LSECs & KCs. a. Unscaled heatmaps of normalized log2 expression values (log2 (RPKM+ 1), & log2 (iBAQ+ 1)) for scavenger receptors (SR) & C-type lectins in the KC & LSEC transcriptomes & proteomes. Underlined: Genes expressed in the transcriptome that were also present in the proteome. b. Absolute abundance of selected SR gene products in the KC & LSEC transcriptomes & proteomes. The bar height reflects good correlation between the transcriptome & proteome data for gene products of Clec4g, Clec4m, Stab1, & Stab2 in both cell types. The abundance of gene products of Marco & Cd5l were well correlated between the KC transcriptome & proteome, while LSECs showed high abundance of these gene products only at mRNA level. c. Immune labeling of non-parenchymal liver cell (NPC) cultures for selected SRs & C-type lectins. NPCs from the 25–45% interface on the Percoll gradient were incubated for 1 h, then fixed 15 min in 4% paraformaldehyde, & double immune-labeled with antibodies to Fc gamma RIIb2 (SE-1; red fluorescence; left column), or CD68 (red fluorescence; right column), & to either stabilin-2 (STAB2; green), mannose receptor (MRC1; green), SR-A1 (green), or SR-B1 (green). Overlap of green & red fluorescence is seen as yellow staining in the overlay images. Antibodies are listed in Table 1. Cell nuclei were stained with DAPI (blue). Arrow heads point to CD68 positive KCs. Antibodies to stabilin-2 & Fc gamma RIIb2 (SE-1) specifically labeled LSECs & the CD68-antibody specifically labeled KCs, whereas positive labeling for the mannose receptor, SR-A1, & SR-B1 was observed in both LSECs & KCs Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33246411), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-68095_mouse-monoclonal-hepatic-sinusoidal-endothelial-cells-antibody-se-1-3102024162186.jpg "Immunocytochemistry/ Immunofluorescence: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Expression of scavenger receptors & immune lectins in rat LSECs & KCs. a. Unscaled heatmaps of normalized log2 expression values (log2 (RPKM+ 1), & log2 (iBAQ+ 1)) for scavenger receptors (SR) & C-type lectins in the KC & LSEC transcriptomes & proteomes. Underlined: Genes expressed in the transcriptome that were also present in the proteome. b. Absolute abundance of selected SR gene products in the KC & LSEC transcriptomes & proteomes. The bar height reflects good correlation between the transcriptome & proteome data for gene products of Clec4g, Clec4m, Stab1, & Stab2 in both cell types. The abundance of gene products of Marco & Cd5l were well correlated between the KC transcriptome & proteome, while LSECs showed high abundance of these gene products only at mRNA level. c. Immune labeling of non-parenchymal liver cell (NPC) cultures for selected SRs & C-type lectins. NPCs from the 25–45% interface on the Percoll gradient were incubated for 1 h, then fixed 15 min in 4% paraformaldehyde, & double immune-labeled with antibodies to Fc gamma RIIb2 (SE-1; red fluorescence; left column), or CD68 (red fluorescence; right column), & to either stabilin-2 (STAB2; green), mannose receptor (MRC1; green), SR-A1 (green), or SR-B1 (green). Overlap of green & red fluorescence is seen as yellow staining in the overlay images. Antibodies are listed in Table 1. Cell nuclei were stained with DAPI (blue). Arrow heads point to CD68 positive KCs. Antibodies to stabilin-2 & Fc gamma RIIb2 (SE-1) specifically labeled LSECs & the CD68-antibody specifically labeled KCs, whereas positive labeling for the mannose receptor, SR-A1, & SR-B1 was observed in both LSECs & KCs Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33246411), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Flow Cytometry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [FITC] [NB110-68095F] - Flow Cytometry: Surface staining of Rat Liver sinusoidal endothelial cells with Mouse anti-Rat Hepatic Sinusoidal Endothelial Cells [FITC] [NB110-68095F] and negative control. Total viable cells were used for analysis. Image courtesy of customer.](https://images.novusbio.com/images/Hepatic-Sinusoidal-Endothelial-Cells-Antibody-SE-1-FITC-Flow-Cytometry-NB110-68095F-img0001.jpg "Flow Cytometry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [FITC] [NB110-68095F] - Flow Cytometry: Surface staining of Rat Liver sinusoidal endothelial cells with Mouse anti-Rat Hepatic Sinusoidal Endothelial Cells [FITC] [NB110-68095F] and negative control. Total viable cells were used for analysis. Image courtesy of customer.")
