HE4/WFDC2 Antibody Summary
| Immunogen |
Peptide with sequence DAEKPGECPQLEPITD, from the N Terminus of the protein sequence according to NP_080599.1. |
| Isotype |
IgG |
| Clonality |
Polyclonal |
| Host |
Goat |
| Gene |
WFDC2 |
| Purity |
Antigen Affinity-purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- Immunohistochemistry 1:10 - 1:500
- Immunohistochemistry-Paraffin
- Peptide ELISA 1:100-1:2000
- Western Blot 1:100-1:2000
|
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles. |
| Buffer |
TBS (pH 7.3), 0.5% BSA |
| Preservative |
0.02% Sodium Azide |
| Concentration |
0.5 mg/ml |
| Purity |
Antigen Affinity-purified |
Alternate Names for HE4/WFDC2 Antibody
Background
The HE4 gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
Publications for HE4/WFDC2 Antibody (NBP2-69988) (0)
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Product General Protocols
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Video Protocols
FAQs for HE4/WFDC2 Antibody (NBP2-69988). (Showing 1 - 1 of 1 FAQ).
-
I would like to build a sandwich ELISA for detection of HE4 levels in human blood. I found there are several HE4 antibodies from your company. Could you please provide me some information among these products, which two antibodies will work well with the HE4 antigen in a sandwich ELISA pair?
- At this time we have not tested any of our HE4 antibodies for use in a Sandwich ELISA. As such, we cannot say with certainty, which will work together as an effective pair. Often times in our experience, choosing a monoclonal antibody for capture, and a polyclonal antibody for detection will yield great results. If you plan on testing any of our HE4 antibodies for use in a Sandwich ELISA then we would encourage you to apply for our Innovators Reward Program. Under the terms of this program, you will be eligible for a full credit in exchange for your data, in the form of an online review, regardless of positive or negative results. Additional Innovator’s Reward information can be found at the following here
Secondary Antibodies
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Isotype Controls
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Additional HE4/WFDC2 Products
Research Areas for HE4/WFDC2 Antibody (NBP2-69988)
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Blogs on HE4/WFDC2
![Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP2-69988] - (5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/HE4-WFDC2-Antibody-Immunohistochemistry-Paraffin-NBP2-69988-img0001.jpg "Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP2-69988] - (5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
