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GPR125 Antibody

Images

 
Western Blot: GPR125 Antibody [NBP1-03561] - Staining of Human Ovary lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: GPR125 Antibody [NBP1-03561] - Bouin's fixed Human Testis stained with anti-GPR125. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: GPR125 Antibody [NBP1-03561] - Staining of Human Cortex. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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GPR125 Antibody Summary

Immunogen
Peptide with sequence CTNKSASSFKNS corresponding to internal region according to NP_660333.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ADGRA3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 3.75 ug/mL
  • Immunohistochemistry-Paraffin 3.75 ug/mL
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.5 - 1.5 ug/mL
Application Notes
WB: Approx. 140 kDa band observed in human ovary lysates (calculated MW of 146 kDa band according to NP_660333.2). An additional band of unknown identity was also consistently observed at 33 kDa. This band was successfully blocked by incubation with the immunizing peptide. GPR125 antibody also validated for IHC-P from a verified customer review.
Theoretical MW
146 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-03561 in the following applications:

Publications
Read Publications using
NBP1-03561 in the following applications:

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33754041).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GPR125 Antibody

  • FLJ38547
  • G protein-coupled receptor 125
  • GPR125
  • PGR21
  • probable G-protein coupled receptor 125
  • TEM5L

Background

GPR125 is an orphan receptor which has a leucine rich repeat (LRR), an immunoglobulin (Ig) domain, and a hormone-binding domain (HBD). The Ig domain shows similarities to motilin andtitin, while the LRR domain shows similarities to LRIG1 and SLIT1-2. ESTs have been isolated primarily from amnion,connective tissue, ear, embryo, eye,ganglion, heart, lung,placenta, and skin libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GPR125 Antibody (NBP1-03561)(3)

Review for GPR125 Antibody (NBP1-03561) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-03561:
Filter by Applications
IHC-P
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin GPR125 NBP1-03561
Enlarge
4
reviewed by:
Marco Seandel
IHC-P Human 05/06/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedTestis
SpeciesHuman

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for GPR125 Antibody (NBP1-03561) (0)

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Secondary Antibodies

 

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Marco Seandel
05/06/2014
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol ADGRA3
Entrez
Uniprot