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Human Glycogen Phosphorylase BB/GPBB ELISA Kit (Colorimetric)

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ELISA: Human Glycogen Phosphorylase BB/GPBB ELISA Kit (Colorimetric) [NBP2-82519] - Samples were spiked with high concentrations of Human Glycogen Phosphorylase BB/GPBB and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human Glycogen Phosphorylase BB/GPBB ELISA Kit (Colorimetric) [NBP2-82519] - Samples were spiked with high concentrations of Human Glycogen Phosphorylase BB/GPBB and diluted with Reference Standard & Sample ...read more
ELISA: Human Glycogen Phosphorylase BB/GPBB ELISA Kit (Colorimetric) [NBP2-82519] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL

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Human Glycogen Phosphorylase BB/GPBB ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human Glycogen Phosphorylase BB/GPBB in samples. No Significant cross-reactivity or interference between Human Glycogen Phosphorylase BB/GPBB and analogues was observed.
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.28
Intra-Assay
CV% < 5.55
Spike Recovery
85-106%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
PYGB

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Glycogen Phosphorylase BB/GPBB ELISA Kit (Colorimetric)

  • Brain glycogen phosphorylase
  • EC 2.4.1.1
  • Glycogen phosphorylase B
  • Glycogen phosphorylase brain form
  • Glycogen Phosphorylase Isoenzyme BB
  • GPBB
  • MGC9213
  • Phosphorylase glycogen brain
  • PYGB

Background

GPBB is found predominantly in the brain. Glycogen phosphorylase is a key enzyme of glycogenolysis, catalyzing the rate determining step in glycogen degradation. The activity of this enzyme is allosterically activated by AMP and alosterically inhibited by ATP, ADP, and glucose 6 phosphate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Human Glycogen Phosphorylase BB/GPBB ELISA Kit (Colorimetric)
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Product PDFs

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  Human Glycogen Phosphorylase BB/GPBB ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol PYGB