| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, IP, IHC-P (-) |
| Clone | D7-FIB |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | DyLight 550 |
| Immunogen | Human foreskin fibroblasts |
| Localization | Cell Membrane |
| Specificity | NB600-777 recognizes a 112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes. D7-FIB has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (3). This product is routinely tested in flow cytometry on the KG1 cell line. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Fibroblasts/Epithelial cells Antibody (NB600-777R)Find related products by research area.
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