![Immunocytochemistry/Immunofluorescence: Fibroblasts Antibody (TE-7) [NBP2-50082] - MRC-5 human embryonic lung fibroblast cell line, fixation with PFA 4%, blocking with PBS 1X + 1% BSA + 0.1% Tween, primary antibody used at 1:50 in blocking buffer, O/N. Secondary antibody: goat anti mouse Alexa Fluor 594 for 1 hour at RT. Signal was detected by fluorescence microscopy at 40X. This image was submitted via customer review.](http://images.novusbio.com/fullsize/Fibroblasts-Antibody-TE-7-Immunocytochemistry-NBP2-50082-img0005.jpg "Immunocytochemistry/Immunofluorescence: Fibroblasts Antibody (TE-7) [NBP2-50082] - MRC-5 human embryonic lung fibroblast cell line, fixation with PFA 4%, blocking with PBS 1X + 1% BSA + 0.1% Tween, primary antibody used at 1:50 in blocking buffer, O/N. Secondary antibody: goat anti mouse Alexa Fluor 594 for 1 hour at RT. Signal was detected by fluorescence microscopy at 40X. This image was submitted via customer review.")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF, IHC |
| Clone | TE-7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.1 mg/ml |
| Immunogen | Whole human thymic stoma cells (Haynes, B.F., et al. (1984). J. Exp. Med. 159(4):1149-1168). |
| Specificity | The antibody reacts with fibroblasts in tissue as well as cultured fibroblasts. On skin sections the antibody reacts with stratum basal epidermal cells. The antigen defined by antibody TE-7 appears to be a marker for mesodermally dervied (mesenchymal) human connective tissue and tumors derived from such tissue, e.g. fibrosarcomas (Haynes, B.F., et al. (1984). J. Exp. Med. 159(4):1149-1168). The antibody is useful for the assessment of thymic epithelial culture contamination with fibroblasts, and the identification of cells of mesodermal origin in the fetus. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS with 1 mg/ml BSA |
| Preservative | 0.07% Sodium Azide |
| Concentration | 0.1 mg/ml |
| Purity | Protein A or G purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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Enlarge |
reviewed by:
Maxime Bourrelle-Langlois |
ICC | Human | 03/22/2018 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
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| Maxime Bourrelle-Langlois 03/22/2018 |
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| Application: | ICC | |
| Species: | Human |
![Immunohistochemistry-Paraffin: Fibroblasts Antibody (TE-7) [NBP2-50082] - Paraffin-embedded gastrocnemius section from a patient with myopathic condition was performed overnight at 4 degrees C using 10 ug/mL.](http://images.novusbio.com/fullsize/Fibroblasts-Antibody-TE-7-Immunohistochemistry-Paraffin-NBP2-50082-img0004.jpg "Immunohistochemistry-Paraffin: Fibroblasts Antibody (TE-7) [NBP2-50082] - Paraffin-embedded gastrocnemius section from a patient with myopathic condition was performed overnight at 4 degrees C using 10 ug/mL.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
