Immunocytochemistry/ Immunofluorescence: Fibroblast Surface Protein Antibody (1B10) [NB100-1845] - Hs68 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:500 ...read more
Product Details
Summary
Reactivity
Hu, MuSpecies Glossary
Applications
Flow, ICC/IF, IHC, Func
Clone
1B10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Fibroblast Surface Protein Antibody (1B10) Summary
Immunogen
cultured human thymic fibroblasts
Localization
Cell Membrane and Lysosome
Marker
Fibroblast Marker
Specificity
This fibroblast antigen has been demonstrated on human synovial, mammary, foreskin and thymic fibroblasts and in malignant fibrosarcoma tissue. The antigen is both cell membrane and lysosome associated.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use in Mouse reported in scientific literature (PMID:33296650)
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
The product is provided as the immunoglobulin fraction of mouse ascites fluid in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA.
Preservative
0.9% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Purity
Unpurified
Background
Overgrowth of epithelial cells by fibroblasts has long been an obstacle to cultivation of normal and abnormal epithelial cells. In addition, apart from the functional significance of cells characteristics, the characterization of cell lines is important for correlation of cultures with tissue origin, identification of the lineage to which the cells belong or the precursor status of the cells. Monoclonal antibodies to fibroblast surface protein should provide a powerful tool for control of fibroblast growth in a variety of human cell culture systems, such as thymic epithelial (TE) cell cultures allowing the growth of highly enriched populations of the TE cells. It is also useful for flow cytometry, immunohistochemistry and immunoblotting.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for Fibroblast Surface Protein Antibody (NB100-1845)(5)
We have publications tested in 2 confirmed species: Human, Mouse.
We have publications tested in 3 applications: FLOW, ICC/IF, IHC-Fr.
FAQs for Fibroblast Surface Protein Antibody (NB100-1845). (Showing 1 - 2 of 2 FAQs).
From your experience with NB100-1845, do you know if it's fibroblast specific? Has this antibody been tested on vascular smooth muscle cells, endothelial cells or dermal/skin fibroblasts?
The antibody recognizes an epitope on primary cultured human fibroblasts, fibroblast cell lines, tissue macrophages and 95% of peripheral blood monocytes. It does not react with vascular smooth muscle cells.
I am currently in search of an antibody for staining of human dermal fibroblasts. Can you recommend me an antibody for this? Is there an antibody that specifically stains only fibroblasts and not mesenchymal stem cells?
We sell a number of antibodies which are suitable for staining fibroblasts. You can see these at the following links: NB100-64932, NB110-15846 and NB100-1845. NB100-64932 recognizes ER-TR7, an antigen that is located in the cytoplasm of reticular fibroblasts and is a component of the extracellular matrix of lymphoid and non-lymphoid organs. The antigen recognized by clone ER-TR7 has not been identified but studies suggest that it is likely to be distinct from laminin, fibronectin, collagen types I-IV, heparin sulphate proteoglycan, entactin and nidogen. NB110-15846 is specific to beta subunit of prolyl 4-hydroxylase. It reacts with fibroblasts and myoepithelial cells, and does not stain lymphocytes, monocytes, dendritic cells or granulocytes. NB100-1845 recognizes an epitope on primary cultured human fibroblasts, fibroblast cell lines, tissue macrophages and 95% of peripheral blood monocytes. It does not react with vascular smooth muscle cells. Unfortunately, we do not have any data regarding the staining of mesenchymal stem cells with any of these antibodies in our QC lab.
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![Immunocytochemistry/Immunofluorescence: Fibroblast Surface Protein Antibody (1B10) [NB100-1845] - Hs68 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:500 Anti-Fibroblast Surface Protein antibody, Mouse monoclonal, Clone: 1B10. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/Fibroblast-Surface-Protein-Antibody-1B10-Immunocytochemistry-Immunofluorescence-NB100-1845-img0001.jpg "Immunocytochemistry/Immunofluorescence: Fibroblast Surface Protein Antibody (1B10) [NB100-1845] - Hs68 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:500 Anti-Fibroblast Surface Protein antibody, Mouse monoclonal, Clone: 1B10. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.")
