Home » ErbB3/Her3 » ErbB3/Her3 Antibodies » ErbB3/Her3 Antibody (66223) [Alexa Fluor® 594]
Novus Biologicals products are now on bio-techne.com

ErbB3/Her3 Antibody (66223) [Alexa Fluor® 594]

 Submit a Review

Images

 
(
Enlarge)
MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ErbB3/Her3 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB3481T, filled histogram) or isotype control antibody (Catalog # IC002T, open histogram). View our protocol for Staining Membrane-associated Proteins.
MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ErbB3/Her3 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB3481T, filled histogram) or isotype control antibody (Catalog # IC002T, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
66223
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 594
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB3481U-100UG
Alexa Fluor 405
FAB3481V-100UG
Alexa Fluor 488
FAB3481G-100
Alexa Fluor 488
FAB3481G-025
Alexa Fluor 532
FAB3481X
Alexa Fluor 647
FAB3481R-100UG
Alexa Fluor 700
FAB3481N-100UG
Alexa Fluor 750
FAB3481S-100UG
Allophycocyanin
FAB3481A-025
Allophycocyanin
FAB3481A-100
Allophycocyanin/Cy7
FAB3481APCCY7
Biotin
FAB3481B
DyLight 350
FAB3481D
DyLight 405
FAB3481E
DyLight 488
FAB3481K
DyLight 550
FAB3481L
DyLight 594
FAB3481M
DyLight 650
FAB3481W
DyLight 680
FAB3481Y
DyLight 755
FAB3481Z
FITC
FAB3481F
HRP
FAB3481H
Janelia Fluor 549
FAB3481I
Janelia Fluor 646
FAB3481J
PE/Atto594
FAB3481PEATT594
PE/Cy5.5
FAB3481PECY55
PE/Cy7
FAB3481PECY7
Phycoerythrin
FAB3481P-100
Phycoerythrin
FAB3481P-025
Unconjugated
MAB3481-100
mFluor Violet 450 SE
FAB3481MFV450
mFluor Violet 500 SE
FAB3481MFV500
mFluor Violet 610 SE
FAB3481MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB3481-100
Carrier Free
MAB3481-SP
Carrier Free
MAB3481-500
Carrier Free
MAB3481-100
Carrier Free
FAB3481A-100
Carrier Free
FAB3481A-025
Carrier Free
FAB3481P-100
Carrier Free
FAB3481P-025
Carrier Free
FAB3481G-100
Carrier Free
FAB3481G-025
Carrier Free
FAB3481V-100UG
Carrier Free
FAB3481U-100UG
Carrier Free
FAB3481S-100UG
Carrier Free
FAB3481R-100UG
Carrier Free
FAB3481N-100UG
100% Guarantee on every product

ErbB3/Her3 Antibody (66223) [Alexa Fluor® 594] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ErbB3/Her3
Ser20-Thr643
Accession # P21860
Specificity
Detects human ErbB3/Her3 in ELISAs. Does not cross-react with recombinant EGF R.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ERBB3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ErbB3/Her3 Antibody (66223) [Alexa Fluor® 594]

  • c-erbB3
  • EC 2.7.10
  • EC 2.7.10.1
  • ErbB3
  • ErbB-3
  • erbB3-S
  • HER3
  • HER3c-erbB-3
  • LCCS2
  • lethal congenital contracture syndrome 2
  • MDA-BF-1
  • MGC88033
  • p180-ErbB3
  • p45-sErbB3
  • p85-sErbB3
  • Proto-oncogene-like protein c-ErbB-3
  • receptor tyrosine-protein kinase erbB-3
  • Tyrosine kinase-type cell surface receptor HER3
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)

Background

ErbB3, also called Her3 (human epidermal growth factor receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. Among ErbB family members, ErbB3 is unique in that it contains a defective kinase domain. ErbB3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric ErbB3 serves as a low affinity receptor for the heregulins (HRG). ErbB3 heterodimerizes with ErbB2 to form a high affinity receptor complex. In contrast, ErbB3 homodimerization or heterodimerization with ErbB4 forms a low affinity heregulin-binding complex. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. The cytoplasmic domain of ErbB3 also contains six consensus binding motifs for the SH2 domain of the regulatory p85 subunit of phosphoinositide 3-kinase (PI 3-kinase, PI3K) as well as one proline-rich consensus binding motif for the SH3 domain of p85. Human ErbB3 consists of 1342 amino acids (aa) with a 19 aa signal sequence, a 624 aa extracellular domain, a 21 aa transmembrane region, and a 678 aa cytoplasmic domain. ErbB3 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation.

  1. Kraus, M.H. et. al. (1989) Proc. Natl. Acad. Sci. USA 86:9193.
  2. Plowman, G.D. et. al. (1990) Proc. Natl. Acad. Sci. USA 87:4905.
  3. Carraway, K.L. 3rd et. al. (1994) J. Biol. Chem. 269:14303.
  4. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  5. Sundaresan, S. et. al. (1998) Endocrinology 139:4756.
  6. Hellyer, N.J. et. al. (1998) Biochem. J. 333:757.
  7. Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
  8. Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
  9. Schlessinger, J. (2000) Cell 103:211.
  10. Daly, R.J. (1999) Growth Factors 16:255.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

EGFR Antibody
[Unconjugated]
AF231
Immunohistochemistry EGFR Antibody [Unconjugated]
Immunocytochemistry EGFR Antibody [Unconjugated]
Western Blot EGFR Antibody [Unconjugated]
(30 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
ErbB2/Her2
[Unconjugated]
1129-ER
SDS-PAGE ErbB2/Her2 [Unconjugated]
Bioactivity ErbB2/Her2 [Unconjugated]
(58 Publications)
Species: Hu
Applications: BA
ErbB4/Her4
Antibody (182803) [Un ...
MAB1131
Immunocytochemistry ErbB4/Her4 Antibody (182803) [Unconjugated]
Immunohistochemistry ErbB4/Her4 Antibody (182803) [Unconjugated]
(5 Publications)
Species: Hu
Applications: ICC, IHC, WB
EGF [Unconjugated]
236-EG
SDS-PAGE EGF [Unconjugated]
Bioactivity EGF [Unconjugated]
Cell Culture EGF [Unconjugated]
(777 Publications)
Species: Hu
Applications: BA
Neuregulin-1/NRG1
Antibody (147724) [Un ...
MAB377
Neuregulin-1/NRG1 Antibody (147724) [Unconjugated]
(84 Publications)
Species: Hu
Applications: WB
AKT [p Ser473]
Antibody [Unconjugate ...
AF887
Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
(49 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
PIK3CA Antibody
NBP2-19804
Western Blot PIK3CA Antibody
Immunohistochemistry-Paraffin PIK3CA Antibody
Western Blot PIK3CA Antibody
(4 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NEU-1/Sialidase-1
Antibody (688215)
MAB6860
Western Blot NEU-1/Sialidase-1 Antibody (688215)
(1 Review)
(2 Publications)
Species: Hu
Applications: IP, WB
PI 3-Kinase p110
beta/PIK3CB Antibody ...
MAB2686
Western Blot PI 3-Kinase p110 beta/PIK3CB Antibody (269020)
(5 Publications)
Species: Hu
Applications: WB
PI 3-Kinase p110
delta Antibody (OTI1D ...
NBP2-46404
Immunohistochemistry PI 3-Kinase p110 delta Antibody (OTI1D11)
Immunohistochemistry PI 3-Kinase p110 delta Antibody (OTI1D11)
Immunohistochemistry PI 3-Kinase p110 delta Antibody (OTI1D11)
(1 Review)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P
PI 3-Kinase p110
gamma Antibody
NBP2-15071
Western Blot PI 3-Kinase p110 gamma Antibody
Immunohistochemistry-Paraffin PI 3-Kinase p110 gamma Antibody
Western Blot PI 3-Kinase p110 gamma Antibody
(3 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
HPRG Antibody
[Unconjugated]
AF1905
HPRG Antibody [Unconjugated]
(2 Publications)
Species: Mu
Applications: WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
Amphiregulin
Antibody
AF262
Neutralization Amphiregulin Antibody
Immunocytochemistry Amphiregulin Antibody
Immunohistochemistry Amphiregulin Antibody
(1 Review)
(35 Publications)
Species: Hu
Applications: ICC, IHC, Neut, WB
ErbB3/Her3
Antibody (66223) [Ale ...
FAB3481T
ErbB3/Her3 Antibody (66223) [Alexa Fluor® 594]
Species: Hu
Applications: Flow

Publications for ErbB3/Her3 Antibody (FAB3481T) (0)

There are no publications for ErbB3/Her3 Antibody (FAB3481T).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ErbB3/Her3 Antibody (FAB3481T) (0)

There are no reviews for ErbB3/Her3 Antibody (FAB3481T). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ErbB3/Her3 Antibody (FAB3481T) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional ErbB3/Her3 Products

Blogs on ErbB3/Her3.

Check out the latest blog posts on ErbB3/Her3.
Hypoxia-Dependent CAR Stabilizing Construct in T cells Improves Solid Tumor Targeting and Efficacy
By Victoria Osinski, PhDDespite advances in the development of cancer immunotherapies, those specifically targeting tumors still remains limited. Currently, there is great interest in utilizing chimeric antigen rece...  Read full blog post.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Goat anti-Mouse IgG Secondary Antibody [HRP]
HAF007
SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.
Mouse IgG1 Isotype Control (MG1)
NBP1-97005-0.5mg

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ErbB3/Her3 Antibody (66223) [Alexa Fluor® 594] and receive a gift card or discount.


Bioinformatics

Gene Symbol ERBB3
Uniprot