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ErbB2/Her2 Antibody (OTI4F10) [Janelia Fluor® 669]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI4F10
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 669

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70653AF350
Alexa Fluor 405
NBP2-70653AF405
Alexa Fluor 488
NBP2-70653AF488
Alexa Fluor 532
NBP2-70653AF532
Alexa Fluor 594
NBP2-70653AF594
Alexa Fluor 647
NBP2-70653AF647
Alexa Fluor 700
NBP2-70653AF700
Alexa Fluor 750
NBP2-70653AF750
Allophycocyanin
NBP2-70653APC
Allophycocyanin/Cy7
NBP2-70653APCCY7
Biotin
NBP2-70653B
CoraFluor 1
NBP2-70653CL1
DyLight 350
NBP2-70653UV
DyLight 405
NBP2-70653V
DyLight 488
NBP2-70653G
DyLight 550
NBP2-70653R
DyLight 594
NBP2-70653DL594
DyLight 650
NBP2-70653C
DyLight 680
NBP2-70653FR
DyLight 755
NBP2-70653IR
FITC
NBP2-70653F
HRP
NBP2-70653H
Janelia Fluor 525
NBP2-70653JF525
Janelia Fluor 549
NBP2-70653JF549
Janelia Fluor 585
NBP2-70653JF585
Janelia Fluor 635
NBP2-70653JF635
Janelia Fluor 646
NBP2-70653JF646
PE
NBP2-70653PE
PE/Atto594
NBP2-70653PEATT594
PE/Cy5.5
NBP2-70653PECY55
PE/Cy7
NBP2-70653PECY7
PerCP
NBP2-70653PCP
Unconjugated
NBP2-01152
mFluor Violet 450 SE
NBP2-70653MFV450
mFluor Violet 500 SE
NBP2-70653MFV500
mFluor Violet 610 SE
NBP2-70653MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-01152
Azide and BSA Free
NBP2-70653
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ErbB2/Her2 Antibody (OTI4F10) [Janelia Fluor® 669] Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 676-1255 of human ERBB2(NP_004439) produced in HEK293T cell.
Specificity
Antibody specificy evaluation by using microarray chip and WB analysis showed that this antibody cross-reacts with CDH1 recombinant protein in overexpression lysates.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ERBB2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for ErbB2/Her2 Antibody (OTI4F10) [Janelia Fluor® 669]

  • CD340 antigen
  • CD340
  • c-erb B2/neu protein
  • EC 2.7.10
  • EGFR2
  • ErbB2
  • HER2
  • HER-2
  • HER2EC 2.7.10.1
  • herstatin
  • Metastatic lymph node gene 19 protein
  • MLN 19
  • MLN19
  • Neu Oncogene
  • NEUHER-2/neu
  • neuroblastoma/glioblastoma derived oncogene homolog
  • NGL
  • NGLTKR1
  • p185erbB2
  • Proto-oncogene c-ErbB-2
  • Proto-oncogene Neu
  • receptor tyrosine-protein kinase erbB-2
  • TKR1
  • Tyrosine kinase-type cell surface receptor HER2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog)
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Background

ErbB2/HER2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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FAQs for ErbB2/Her2 Antibody (NBP2-70653JF669). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
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Bioinformatics

Gene Symbol ERBB2