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ErbB2/Her2 Antibody (Hu5) [Unconjugated]

Images

 
MCF-7 human cell line was stained with Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Monoclonal Antibody (Catalog # MAB9589, filled histogram) or Human IgG Control Antibody (1-001-A, open ...read more
MDA-MB-453 human cell line was stained with Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Monoclonal Antibody (Catalog # MAB9589, filled histogram) or Human IgG Control Antibody (1-001-A, open ...read more
Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Monoclonal Antibody (Catalog # MAB9589) inhibits proliferation in the SK‑BR‑3 human breast cancer cell line as measured by Resazurin (Catalog # ...read more
ADCC assays with SKOV-3 and Raji targets. NK cells were cultured as described in the adjacent figure for 13 days. Prior to setting up the ADCC assay, CD56+CD16+ levels were ascertained to be >30% on NKs using Human Fc ...read more
Biosimilar antibody development at Bio-Techne includes in silico design and engineering to generate both light chain (lc) and heavy chain (hc) sequences for each antibody. After transfection of the dual expression ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, B/N, CyTOF-ready
Clone
Hu5
Clonality
Monoclonal
Host
Human
Conjugate
Unconjugated

Order Details

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ErbB2/Her2 Antibody (Hu5) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody For Research Use Only
Immunogen
Human ErbB2/Her2
Specificity
Detects human ErbB2/Her2 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Trastuzumab. This product is for research use only.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Human
Purity Statement
Protein A or G purified from cell culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Inhibition of Cell Growth 15-75 ng/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB9589 in the following applications:

Publications
Read Publications using
MAB9589 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ErbB2/Her2 Antibody (Hu5) [Unconjugated]

  • CD340 antigen
  • CD340
  • c-erb B2/neu protein
  • EC 2.7.10
  • EGFR2
  • ErbB2
  • HER2
  • HER-2
  • HER2EC 2.7.10.1
  • herstatin
  • Metastatic lymph node gene 19 protein
  • MLN 19
  • MLN19
  • Neu Oncogene
  • NEUHER-2/neu
  • neuroblastoma/glioblastoma derived oncogene homolog
  • NGL
  • NGLTKR1
  • p185erbB2
  • Proto-oncogene c-ErbB-2
  • Proto-oncogene Neu
  • receptor tyrosine-protein kinase erbB-2
  • TKR1
  • Tyrosine kinase-type cell surface receptor HER2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog)
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Background

Trastuzumab is a biosimilar directed against the extracellular domain of Her2 (ErbB2). This product constitutes the immunogen used for the anti-Idiotype catalog numbers MAB9547 and MAB95471. The biotinylated antibody (catalog number FAB9589B) can be used in flow cytometry or various assay formats to measure Her2 expression and can be detected by conjugated streptavidin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ErbB2/Her2 Antibody (MAB9589)(4)

We have publications tested in 3 confirmed species: Human, Staphylococcus, Streptococcus.

We have publications tested in 4 applications: Flow Cytometry, Immunoprecipitation, Neutralization Control, Western Blot.


Filter By Application
Flow Cytometry
(1)
Immunoprecipitation
(1)
Neutralization Control
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(2)
Staphylococcus
(1)
Streptococcus
(1)
All Species

Review for ErbB2/Her2 Antibody (MAB9589) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB9589:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IF Human 06/17/2021
View

Summary

ApplicationImmunofluorescence
Sample Testedhuman HEK293T cells
SpeciesHuman
LotCKIXO419101

Comments

CommentsImmunofluorescence on 4% PFA fixed cells after Triton X-100 permeabilization. The staining was done on Human HEK293 cell line that was transfected with a viral vector to express HER2.
***Novus Innovators Program - new species or application used on a primary antibody.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ErbB2/Her2 Antibody (MAB9589). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
    • A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22

Secondary Antibodies

 

Isotype Controls

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By Victoria Osinski, PhDWhat’s “Natural” About Natural Killer (NK) Cells?For immunologists, the term cytotoxicity often conjures up images of an army of antigen specific CD8+ T cells deploying to ...  Read full blog post.

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Recent Reviews

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Verified Customer
06/17/2021
Application: IF
Species: Human