This mAb is a very useful tool for clinical and experimental allergy studies. BMK-13 is a pan-eosinophil marker in both frozen sections and paraffin embedded tissues. It stains in bronchial sections 98% of the periphere blood eosinophils. Further, the antibody is most useful for immunohistochemistry on frozen sections is the APAAP method. BMK-13 also binds to eosinophils in paraffin-embedded tissue. A 1:30 dilution in PBS is recommended. Incubation time: 1 hour at RT. Enzymatic pretreatment is necessary. This antibody is suitable for blood smears (cytospins) if fixed with methanol-acetone mixture (1:1, v/v). It is unlikely, without permeabilization, that the BMK-13 is suitable for the flow cytometry because BMK-13 stains intracellular, stored proteins.
Publications
Read Publications using NBP1-42140 in the following applications:
Use in Rhesus Macaque reported in scientific literature (PMID:32128254). Canine reactivity reported in scientific literature (PMID: 29920692). Monkey reactivity reported in scientific literature (PMID: 30626745). Mouse reactivity reported in scientific literature (PMID: 31643033, 31626785).
Packaging, Storage & Formulations
Storage
Store at 4C. Do not freeze.
Buffer
PBS, 0.1% BSA
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein A or G purified
Alternate Names for Eosinophil Antibody (BMK-13)
BMPG
Bone marrow proteoglycan
EMBP
Eosinophil granule major basic protein
Eosinophil major basic protein
MBP
MGC14537
MPG
Natural killer cell activator
Pregnancy associated major basic protein
PRG 2
Proteoglycan 2 bone marrow
Proteoglycan 2 preproprotein
Proteoglycan 2
Proteoglycan2
Background
PRG2 is a cytotoxin and helminthotoxin. Also induces noncytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA. In pregnancy serum, the proform exists as a disulfide-linked 2:2 heterotetramer with PAPPA, as a disulfide-linked 2:2 heterotetramer with AGT, and as a complex (probably a 2:2:2 heterohexamer) with AGT and C3dg.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for Eosinophil Antibody (NBP1-42140)(7)
We have publications tested in 4 confirmed species: Mouse, Canine, Monkey, Rhesus Macaque.
We have publications tested in 3 applications: ELISA, IF/IHC, IHC-P.
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")