Envelope Antibody (1044526) [Unconjugated] Summary
| Immunogen |
Chinese Hamster Ovary cell line CHO-derived human SARS2-CoV-2 Envelope protein
Thr35-Val75
Accession # YP_009724392 |
| Specificity |
Detects human SARS-CoV-2 Envelope protein in direct ELISAs. |
| Source |
N/A |
| Isotype |
IgG2b |
| Clonality |
Monoclonal |
| Host |
Mouse |
| Purity Statement |
Protein A or G purified from hybridoma culture supernatant |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
|
| Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Reconstitution Instructions |
Reconstitute at 0.5 mg/mL in sterile PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Envelope Antibody (1044526) [Unconjugated]
Background
The SARS-CoV-2 Envelope protein is one of the
four major structural proteins of severe acute respiratory syndrome
coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). The envelope
protein is the smallest of the four structural proteins, which also includes
the membrane protein, spike protein, and nucleocapsid protein (1, 2). The
envelope protein is synthesized as a 75 amino acid protein with a theoretical
molecular weight of approximately 8.4 kDa (2, 3). Furthermore, the envelope
protein of SARS-CoV-2 has 94.7% sequence identity and 97.4% sequence similarity
to the envelope protein of SARS-CoV (2). Structurally, the envelope protein is
a membrane protein with a N-terminal domain, an alpha-helical transmembrane
domain, and a hydrophilic C-terminal domain (1,4). The envelope protein has
multiple functions in viral replication including viral assembly, release, and
pathogenesis (2,4). Additionally, the SARS-CoV-2 envelope protein has ion
channel activity and functions as a viroporin with a role in virion trafficking
(2, 4). Coronaviruses lacking the envelope protein are shown to have reduced
viral titer and slowed or defective maturation, indicative of a role in virus
production and growth (4).
- Malik, Y.A. (2020) Properties of
Coronavirus and SARS-CoV-2. The Malaysian Journal of Pathology.
- Yoshimoto F.K. (2020). The Proteins of
Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the
Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4.
- Uniprot (P0DTC4).
- J. Alsaadi, E.A. & Jones, I. M. (2019).
Membrane binding proteins of coronaviruses. Future Virology. https://doi.org/10.2217/fvl-2018-0144.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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