![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] - Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope. Fusion nuclear protein (red) stained with purified DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] (detection by Goat anti-mouse IgG1 Alexa Fluor 594).](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-F-tag-01-Immunocytochemistry-Immunofluorescence-NB500-554-img0004.jpg "Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] - Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope. Fusion nuclear protein (red) stained with purified DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] (detection by Goat anti-mouse IgG1 Alexa Fluor 594).")
| Reactivity | ETSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | F-tag-01 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | DYKDDDDK Epitope Tag Antibody (F-tag-01) was made to a Synthetic peptide: DYKDDDDK conjugated to KLH. |
| Specificity | DYKDDDDK Epitope Tag Antibody (F-tag-01) recognizes DDDDK-tagged proteins in all species. The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 1.01 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.4) |
| Preservative | 0.9% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A purified |
| Publication using NB500-554 | Applications | Species |
|---|---|---|
| Darwish GH, Massey M, Daudet G et al. Tetrameric Antibody Complexes and Affinity Tag Peptides for the Selective Immobilization and Imaging of Single Quantum Dots Bioconjugate chemistry 2023-05-27 [PMID: 37243625] (Epitope Tag) | Epitope Tag |
Secondary Antibodies |
Isotype Controls |
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Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto... Read full blog post. |
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Hoist the DYKDDDDK Epitope Tag The DYKDDDDK Epitope Tag antibody was raised against a short, artificial sequence that is widely and commonly used as a protein fusion tag. It is pervasively present in a vast amount of scientific studies, and is a very powerful investigative research... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] - Immunocytochemistry (confocal microscopy) of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] stained with DAPI (blue).](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-F-tag-01-Immunocytochemistry-Immunofluorescence-NB500-554-img0002.jpg "Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] - Immunocytochemistry (confocal microscopy) of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] stained with DAPI (blue).")
![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] - Immunocytochemistry (confocal microscopy) of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554]. Merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-F-tag-01-Immunocytochemistry-Immunofluorescence-NB500-554-img0003.jpg "Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] - Immunocytochemistry (confocal microscopy) of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554]. Merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue).")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")