![Immunohistochemistry-Paraffin: DOG1/TMEM16A Antibody (DG1/1486) - Azide and BSA Free [NBP2-54484] - Formalin-fixed, paraffin-embedded human GIST stained with DOG-1 Monoclonal Antibody (DG1/1486).](http://images.novusbio.com/fullsize/DOG1-TMEM16A-Antibody-DG1-1486-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54484-img0001.jpg "Immunohistochemistry-Paraffin: DOG1/TMEM16A Antibody (DG1/1486) - Azide and BSA Free [NBP2-54484] - Formalin-fixed, paraffin-embedded human GIST stained with DOG-1 Monoclonal Antibody (DG1/1486).")
| Reactivity | HuSpecies Glossary |
| Applications | IHC, MA |
| Clone | DG1/1486 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53163). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant human canine1/TMEM16A protein fragment (around aa 2-101) (exact sequence is proprietary) (Uniprot: Q5XX6) |
| Localization | Cell Surface and Cytoplasm |
| Marker | Gastrointestinal Stromal Tumor Marker |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ANO1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 114 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ANO1 |
![Protein Array: DOG1/TMEM16A Antibody (DG1/1486) - Azide and BSA Free [NBP2-54484] - Analysis of Protein Array containing more than 19,000 full-length human proteins using DOG1/TMEM16A Antibody (DG1/1486). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/DOG1-TMEM16A-Antibody-DG1-1486-Azide-and-BSA-Free-Protein-Array-NBP2-54484-img0002.jpg "Protein Array: DOG1/TMEM16A Antibody (DG1/1486) - Azide and BSA Free [NBP2-54484] - Analysis of Protein Array containing more than 19,000 full-length human proteins using DOG1/TMEM16A Antibody (DG1/1486). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.")
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