| Reactivity | HuSpecies Glossary |
| Applications | B/N, CyTOF-ready, ELISA(Cap), Flow |
| Clone | 33036 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | E. coli-derived recombinant human CXCL10/IP-10/CRG-2 Val22-Pro98 Accession # P02778.2 |
| Specificity | Detects human CXCL10/IP-10/CRG-2 in ELISAs. |
| Source | N/A |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CXCL10 |
| Purity Statement | Protein A or G purified from ascites |
| Endotoxin Note | <0.10 EU per 1 μg of the antibody by the LAL method. |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA Detection: Human CXCL10/IP-10/CRG-2 Biotinylated Antibody (Catalog number BAF266) Standard: Recombinant Human CXCL10/IP-10/CRG-2 (Catalog number 266-IP) |
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| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Reconstitution Instructions | Reconstitute at 0.5 mg/mL in sterile PBS. |
CXCL10 was originally identified as an IFN-gamma -inducible gene in monocytes, fibroblasts and endothelial cells. It has since been shown that CXCL10 mRNA is also induced by LPS, IL-1 beta , TNF-alpha , IL-12, and viruses. Additional cell types that have been shown to express CXCL10 include activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. CXCL10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human CXCL10, CRG-2, has been cloned and shown to share approximately 67% amino acid sequence identity with human CXCL10. Human CXCL10 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted protein. The amino acid sequence of CXCL10 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes. CXCL10 has been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent antitumor effect. A chemokine receptor specific for CXCL10 and Mig has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.
Secondary Antibodies |
Isotype Controls |
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